General Stability | Organism |
---|---|
immobilized enzyme, higher stability than soluble enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-aminopenicillanic acid | non-competitive inhibition | Escherichia coli | |
6-aminopenicillanic acid | non-competitive inhibition | Priestia megaterium | |
benzyl penicillin | - |
Escherichia coli | |
phenylacetic acid | competitive inhibition | Escherichia coli | |
phenylacetic acid | competitive inhibition | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
benzylpenicillin | - |
Escherichia coli | |
4.5 | - |
benzylpenicillin | - |
Priestia megaterium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Priestia megaterium | - |
- |
intracellular | - |
Escherichia coli | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Escherichia coli |
71000 | - |
sedimentation equilibrium | Escherichia coli |
120000 | - |
sedimentation equilibrium | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Priestia megaterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
48 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, freeze dried pure enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-furylmethylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + furan-2-yl-acetic acid | - |
? | |
2-thienylmethylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + sulfo-(5-sulfothiophen-2-yl)-acetic acid | - |
? | |
benzylpenicilloic acid + H2O | - |
Escherichia coli | ? | - |
? | |
D-alpha-aminobenzylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + DL-alpha-aminophenylacetic acid | - |
? | |
DL-alpha-hydroxybenzylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + DL-alpha-hydroxyphenylacetic acid | - |
? | |
isobutoxymethylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + carbonic acid isopropyl ester | - |
? | |
additional information | overview on best substrates for similar organisms | Escherichia coli | ? | - |
? | |
additional information | overview on best substrates for similar organisms | Priestia megaterium | ? | - |
? | |
N-phenylacetyl-DL-leucine + H2O | - |
Escherichia coli | ? | - |
? | |
N-phenylacetyl-L-alpha-aminophenylacetic acid + H2O | - |
Escherichia coli | ? | - |
? | |
N-phenylacetylglycine + H2O | - |
Escherichia coli | ? | - |
? | |
n-propoxymethylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + carbonic acid monopropyl ester | - |
? | |
p-hydroxybenzylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + p-hydroxyphenylacetic acid | - |
? | |
penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanate + phenylacetic acid | - |
r | |
penicillin G + H2O | - |
Priestia megaterium | 6-aminopenicillanate + phenylacetic acid | - |
? | |
phenylacetamide + H2O | - |
Escherichia coli | ? | - |
? | |
phenylacetamide + H2O | - |
Priestia megaterium | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
overview on values for similar organisms | Escherichia coli |
additional information | - |
overview on values for similar organisms | Priestia megaterium |
37 | - |
generally chosen because enzyme unstable at 45°C and 55°C | Escherichia coli |
37 | - |
generally chosen because enzyme unstable at 45°C and 55°C | Priestia megaterium |
45 | - |
benzylpenicillin | Priestia megaterium |
55 | - |
benzylpenicillin | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
overview on values for similar organisms | Escherichia coli |
additional information | - |
overview on values for similar organisms | Priestia megaterium |
8.2 | - |
- |
Escherichia coli |
8.5 | - |
- |
Priestia megaterium |