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Literature summary for 3.5.1.105 extracted from

  • Mine, S.; Niiyama, M.; Hashimoto, W.; Ikegami, T.; Koma, D.; Ohmoto, T.; Fukuda, Y.; Inoue, T.; Abe, Y.; Ueda, T.; Morita, J.; Uegaki, K.; Nakamura, T.
    Expression from engineered Escherichia coli chromosome and crystallographic study of archaeal N,N'-diacetylchitobiose deacetylase (2014), FEBS J., 281, 2584-2596.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the soluble recombinant protein in Escherichia coli Pyrococcus horikoshii
expression of the soluble recombinant protein in Escherichia coli Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure is determined at resolutions of 1.54 A Pyrococcus furiosus
crystal structure is determined at resolutions of 2.0 A Pyrococcus horikoshii

Inhibitors

Inhibitors Comment Organism Structure
Zinc
-
Pyrococcus furiosus
Zinc the catalytic zinc that is situated at the end of the intermolecular cleft is coordinated by three side chain ligands from His44, Asp47, and His155, and by a phosphate ion derived from the crystallization reservoir solution Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U3V1
-
-
Pyrococcus horikoshii O58235
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus horikoshii
-
Pyrococcus furiosus

Subunits

Subunits Comment Organism
hexamer comprised of two trimers. An intermolecular cleft is formed by two polypeptides in the trimeric assembly Pyrococcus horikoshii
hexamer comprised of two trimers. An intermolecular cleft is formed by two polypeptides in the trimeric assembly Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
N,N'-diacetylchitobiose deacetylase
-
Pyrococcus horikoshii
N,N'-diacetylchitobiose deacetylase
-
Pyrococcus furiosus
PF0354 locus name Pyrococcus furiosus
PH0499 locus name Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
thermostable up to Pyrococcus horikoshii
85
-
thermostable up to Pyrococcus furiosus