Cloned (Comment) | Organism |
---|---|
expression of the soluble recombinant protein in Escherichia coli | Pyrococcus horikoshii |
expression of the soluble recombinant protein in Escherichia coli | Pyrococcus furiosus |
Crystallization (Comment) | Organism |
---|---|
crystal structure is determined at resolutions of 1.54 A | Pyrococcus furiosus |
crystal structure is determined at resolutions of 2.0 A | Pyrococcus horikoshii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zinc | - |
Pyrococcus furiosus | |
Zinc | the catalytic zinc that is situated at the end of the intermolecular cleft is coordinated by three side chain ligands from His44, Asp47, and His155, and by a phosphate ion derived from the crystallization reservoir solution | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U3V1 | - |
- |
Pyrococcus horikoshii | O58235 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
- |
Pyrococcus furiosus |
Subunits | Comment | Organism |
---|---|---|
hexamer | comprised of two trimers. An intermolecular cleft is formed by two polypeptides in the trimeric assembly | Pyrococcus horikoshii |
hexamer | comprised of two trimers. An intermolecular cleft is formed by two polypeptides in the trimeric assembly | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
N,N'-diacetylchitobiose deacetylase | - |
Pyrococcus horikoshii |
N,N'-diacetylchitobiose deacetylase | - |
Pyrococcus furiosus |
PF0354 | locus name | Pyrococcus furiosus |
PH0499 | locus name | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
thermostable up to | Pyrococcus horikoshii |
85 | - |
thermostable up to | Pyrococcus furiosus |