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Literature summary for 3.5.1.105 extracted from

  • Mine, S.; Ikegami, T.; Kawasaki, K.; Nakamura, T.; Uegaki, K.
    Expression, refolding, and purification of active diacetylchitobiose deacetylase from Pyrococcus horikoshii (2012), Protein Expr. Purif., 84, 265-269.
    View publication on PubMed
Search for more literature for 3.5.1.105

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed as inclusion bodies in Escherichia coli Rosetta (DE3) pLys. The insoluble inclusion body is solubilized and reactivated through a refolding procedure Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31595
-
 6 * 31595, calculated from sequence Pyrococcus horikoshii
174000
-
 gel filtration Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranose + H2O Pyrococcus horikoshii i.e. N,N'-diacetylchitobiose, the enzyme is involved in chitin catabolic pathway 2-acetamido-4-O-(2-amino-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-D-glucose + acetate
-
 ?
2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranose + H2O Pyrococcus horikoshii DSM 12428 i.e. N,N'-diacetylchitobiose, the enzyme is involved in chitin catabolic pathway 2-acetamido-4-O-(2-amino-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-D-glucose + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58235
-
-
Pyrococcus horikoshii DSM 12428 O58235
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranose + H2O i.e. N,N'-diacetylchitobiose, the enzyme is involved in chitin catabolic pathway Pyrococcus horikoshii 2-acetamido-4-O-(2-amino-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-D-glucose + acetate
-
 ?
2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranose + H2O i.e. N,N'-diacetylchitobiose, the deacetylation site is specific to the nonreducing end residue of (GlcNAc)(2). The enzyme can also deacetylate N-acetyl-D-glucosamine (cf. EC 3.5.1.33) Pyrococcus horikoshii 2-acetamido-4-O-(2-amino-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-D-glucose + acetate
-
 ?
2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranose + H2O i.e. N,N'-diacetylchitobiose, the enzyme is involved in chitin catabolic pathway Pyrococcus horikoshii DSM 12428 2-acetamido-4-O-(2-amino-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-D-glucose + acetate
-
 ?
2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranose + H2O i.e. N,N'-diacetylchitobiose, the deacetylation site is specific to the nonreducing end residue of (GlcNAc)(2). The enzyme can also deacetylate N-acetyl-D-glucosamine (cf. EC 3.5.1.33) Pyrococcus horikoshii DSM 12428 2-acetamido-4-O-(2-amino-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-D-glucose + acetate
-
 ?

Subunits

Subunits Comment Organism
homohexamer 6 * 31595, calculated from sequence Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
diacetylchitobiose deacetylase
-
 Pyrococcus horikoshii
Ph-Dac
-
 Pyrococcus horikoshii
PH0499 locus name Pyrococcus horikoshii