Literature summary for 3.5.1.1 extracted from

Maggi, M.; Mittelman, S.D.; Parmentier, J.H.; Colombo, G.; Meli, M.; Whitmire, J.M.; Merrell, D.S.; Whitelegge, J.; Scotti, C.
A protease-resistant Escherichia coli asparaginase with outstanding stability and enhanced anti-leukaemic activity in vitro (2017), Sci. Rep., 7, 14479 .

Search for more literature for 3.5.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
N24S	mutant shows completely preserved asparaginase and glutaminase activities, long-term storage stability, improved thermal parameters, and good resistance to proteases derived from leukaemia cells. The mutant displays a modification in the hydrogen bond network related to residue 24, and a general rigidification of the monomer as compared to wild-type	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.03	-	L-asparagine	wild-type, pH not specified in the publication, temperature not specified in the publication	Escherichia coli
0.03	-	L-asparagine	mutant N24S, pH not specified in the publication, temperature not specified in the publication	Escherichia coli
3.95	-	L-glutamine	wild-type, pH not specified in the publication, temperature not specified in the publication	Escherichia coli
4.14	-	L-glutamine	mutant N24S, pH not specified in the publication, temperature not specified in the publication	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P00805	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
103	-	mutant N24S, pH not specified in the publication, temperature not specified in the publication	Escherichia coli
105	-	wild-type, pH not specified in the publication, temperature not specified in the publication	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-asparagine + H2O	-	Escherichia coli	L-aspartate + NH3	-	?
L-glutamine + H2O	reaction of EC 3.5.1.2	Escherichia coli	L-glutamate + NH3	-	?

Synonyms

Synonyms	Comment	Organism
AnsB	-	Escherichia coli
L-asparaginase 2	-	Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
49	-	melting temperature, wild-type	Escherichia coli
59	-	melting temperature, mutant N24S	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.51	-	L-glutamine	wild-type, pH not specified in the publication, temperature not specified in the publication	Escherichia coli
0.53	-	L-glutamine	mutant N24S, pH not specified in the publication, temperature not specified in the publication	Escherichia coli
58.8	-	L-asparagine	mutant N24S, pH not specified in the publication, temperature not specified in the publication	Escherichia coli
59.8	-	L-asparagine	wild-type, pH not specified in the publication, temperature not specified in the publication	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.13	-	L-glutamine	wild-type, pH not specified in the publication, temperature not specified in the publication	Escherichia coli
0.13	-	L-glutamine	mutant N24S, pH not specified in the publication, temperature not specified in the publication	Escherichia coli
1782	-	L-asparagine	mutant N24S, pH not specified in the publication, temperature not specified in the publication	Escherichia coli
1975	-	L-asparagine	wild-type, pH not specified in the publication, temperature not specified in the publication	Escherichia coli

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Release 2023.1 (January 2023)