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Literature summary for 3.5.1.1 extracted from

  • El-Naggar, N.E.; Deraz, S.F.; Soliman, H.M.; El-Deeb, N.M.; El-Ewasy, S.M.
    Purification, characterization, cytotoxicity and anticancer activities of L-asparaginase, anti-colon cancer protein, from the newly isolated alkaliphilic Streptomyces fradiae NEAE-82 (2016), Sci. Rep., 6, 32926 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.1

Application

Application Comment Organism
medicine L-asparaginase may significantly alter the interactions between microvascular endothelial cells, colon cancer cells, and components, resulting in colon cancer cell injury Streptomyces fradiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 L-asparagine pH 8.6, 37°C Streptomyces fradiae

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae
-
-
-
Streptomyces fradiae NEAE-82
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces fradiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30.6
-
 pH 8.6, 37°C Streptomyces fradiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Streptomyces fradiae L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Streptomyces fradiae NEAE-82 L-aspartate + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Streptomyces fradiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Streptomyces fradiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life 185 min Streptomyces fradiae
60
-
 half-life 179 min Streptomyces fradiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Streptomyces fradiae