Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.1 extracted from

  • Dash, C.; Mohapatra, S.; Maiti, P.
    Optimization, purification, and characterization of L-asparaginase from Actinomycetales bacterium BkSoiiA (2016), Prep. Biochem. Biotechnol., 46, 1-7 .
    View publication on PubMed
Search for more literature for 3.5.1.1

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 10 mM, 116% of initial activity Actinomycetales bacterium

Application

Application Comment Organism
synthesis production and optimization of growth conditions results in submerged fermentation in modified M9 medium with yeast extract and fructose as carbon and nitrogen sources, respectively, at pH 8.0, incubated for 120 h at 30°C Actinomycetales bacterium

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 10 mM, 12% residual activity Actinomycetales bacterium
Ca2+ 10 mM, 28% residual activity Actinomycetales bacterium
Cu2+ 10 mM, 23% residual activity Actinomycetales bacterium
Fe2+ 10 mM, 44% residual activity Actinomycetales bacterium
Hg2+ 10 mM, 33% residual activity Actinomycetales bacterium
Li+ 10 mM, 17% residual activity Actinomycetales bacterium
additional information not inhibitory: EDTA at 1-50 mM Actinomycetales bacterium
Zn2+ 10 mM, 34% residual activity Actinomycetales bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 L-asparagine pH not specified in the publication, temperature not specified in the publication Actinomycetales bacterium

Organism

Organism UniProt Comment Textmining
Actinomycetales bacterium KC573069 partial
-
Actinomycetales bacterium BkSoiiA KC573069 partial
-

Purification (Commentary)

Purification (Comment) Organism
-
 Actinomycetales bacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
204
-
 pH not specified in the publication, temperature not specified in the publication Actinomycetales bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Actinomycetales bacterium L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Actinomycetales bacterium BkSoiiA L-aspartate + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Actinomycetales bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Actinomycetales bacterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9
-
 Actinomycetales bacterium