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Literature summary for 3.5.1.1 extracted from

  • Rahimzadeh, M.; Poodat, M.; Javadpour, S.; Qeshmi, F.; Shamsipour, F.
    Purification, characterization and comparison between two new L-asparaginases from Bacillus PG03 and Bacillus PG04 (2016), Open Biochem. J., 10, 35-45 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3
-
 L-asparagine pH 7.2, 37°C Bacillus sp. (in: Bacteria)
23.8
-
 L-asparagine pH 7.2, 37°C Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) PG03
-
-
-
Bacillus sp. (in: Bacteria) PG04
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Bacillus sp. (in: Bacteria) L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Bacillus sp. (in: Bacteria) PG04 L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Bacillus sp. (in: Bacteria) PG03 L-aspartate + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Bacillus sp. (in: Bacteria)
? x * 30000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Bacillus sp. (in: Bacteria)
40
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 half-life 34.6 min Bacillus sp. (in: Bacteria)
37
-
 half-life 69 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus sp. (in: Bacteria)
7
-
-
 Bacillus sp. (in: Bacteria)

Expression

Organism Comment Expression
Bacillus sp. (in: Bacteria) maximum productivity is obtained with asparagine as the nitrogen source at pH 7 and 48 h after cultivation up