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Literature summary for 3.5.1.1 extracted from
- Glutaminase free l-asparaginase from Vibrio cholerae Heterologous expression, purification and biochemical characterization (2018), Int. J. Biol. Macromol., 111, 129-138 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Vibrio cholerae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
L-asparagine | pH 7.0, 37°C | Vibrio cholerae |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 132000 | - |
gel filtration | Vibrio cholerae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio cholerae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Vibrio cholerae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2120 | - |
pH 7.0, 37°C | Vibrio cholerae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Vibrio cholerae | L-aspartate + NH3 | - |
? | |
| additional information | no L-glutaminase activity | Vibrio cholerae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 36600, SDS-PAGE | Vibrio cholerae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Vibrio cholerae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 45 | above 45°C sharp decline in activity | Vibrio cholerae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
half-life 4157 min | Vibrio cholerae |
| 40 | - |
half-life 751 min | Vibrio cholerae |
| 45 | - |
half-life 13.6 min | Vibrio cholerae |
| 50 | - |
half-life 2.4 min | Vibrio cholerae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4424 | - |
L-asparagine | pH 7.0, 37°C | Vibrio cholerae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Vibrio cholerae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | - |
Vibrio cholerae |
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