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Literature summary for 3.5.1.1 extracted from

  • Labrou, N.E.; Muharram, M.M.
    Biochemical characterization and immobilization of Erwinia carotovora l-asparaginase in a microplate for high-throughput biosensing of L-asparagine (2016), Enzyme Microb. Technol., 92, 86-93 .
    View publication on PubMed
Search for more literature for 3.5.1.1

Application

Application Comment Organism
analysis assembly a microplate-based biosensor for the determination of L-asparagine in biological samples. The enzyme is immobilized by crosslinking with glutaraldehyde in a microplate in 96-well format. The sensing is based on the colorimetric measurement of ammonia formation using the Nessler's reagent. The sensor enables monitoring of L-asparagine levels in serum and foods samples in the concentration range 10-200 microM, with a detection limit of 10 microM for L-asparagine Pectobacterium carotovorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 L-asparagine pH 8.0, 25°C Pectobacterium carotovorum
0.3
-
 succinamic acid pH 8.0, 25°C Pectobacterium carotovorum
0.5
-
 Nalpha-acetyl-L-asparagine pH 8.0, 25°C Pectobacterium carotovorum
5.2
-
 L-glutamine pH 8.0, 25°C Pectobacterium carotovorum

Organism

Organism UniProt Comment Textmining
Pectobacterium carotovorum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
single-step procedure Pectobacterium carotovorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Pectobacterium carotovorum L-aspartate + NH3
-
 ?
L-glutamine + H2O reaction of EC 3.5.1.2 Pectobacterium carotovorum L-glutamate + NH3
-
 ?
additional information no substrate: D-asparagine, beta-alanine amide Pectobacterium carotovorum ?
-
 ?
Nalpha-acetyl-L-asparagine + H2O
-
 Pectobacterium carotovorum Nalpha-acetyl-L-aspartate + NH3
-
 ?
succinamic acid + H2O
-
 Pectobacterium carotovorum succinate + NH3
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.42
-
 L-glutamine pH 8.0, 25°C Pectobacterium carotovorum
4.1
-
 succinamic acid pH 8.0, 25°C Pectobacterium carotovorum
4.1
-
 Nalpha-acetyl-L-asparagine pH 8.0, 25°C Pectobacterium carotovorum
55.2
-
 L-asparagine pH 8.0, 25°C Pectobacterium carotovorum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.42
-
 L-glutamine pH 8.0, 25°C Pectobacterium carotovorum
8.2
-
 Nalpha-acetyl-L-asparagine pH 8.0, 25°C Pectobacterium carotovorum
13.7
-
 succinamic acid pH 8.0, 25°C Pectobacterium carotovorum
788.6
-
 L-asparagine pH 8.0, 25°C Pectobacterium carotovorum