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Literature summary for 3.5.1.1 extracted from
- Purification and characterization of glutaminase free asparaginase from Pseudomonas otitidis Induce apoptosis in human leukemia MOLT-4 cells (2016), Biochimie, 121, 38-51 .
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the anticancer activity of purified asparaginase is comparable or higher than that of commercial Escherichia coli asparaginase. The purified enzyme induces apoptotic cell death in dose-dependent manner, probybly via activation of anintrinsic apoptotic pathway. The purified enzyme is nontoxic for human noncancerous FR-2 cells and human blood lymphocytes | Pseudomonas otitidis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 150 mM, about 75% of initial activity | Pseudomonas otitidis |  |
| Cd2+ | 10 mM, loss of activity | Pseudomonas otitidis | |
| EDTA | 5 mM, about 85% residual activity | Pseudomonas otitidis | |
| Fe3+ | 100 mM, about 55% of initial activity | Pseudomonas otitidis | |
| Hg2+ | 100 mM, loss of activity | Pseudomonas otitidis | |
| Mg2+ | 40 mM, about 30% of initial activity | Pseudomonas otitidis | |
| Mn2+ | 100 mM, about 75% of initial activity | Pseudomonas otitidis | |
| Ni2+ | 10 mM, loss of activity | Pseudomonas otitidis | |
| SDS | 2 mM, about 55% residual activity | Pseudomonas otitidis | |
| Thiourea | 1 mM, about 85% residual activtiy | Pseudomonas otitidis | |
| Zn2+ | 100 mM, about 60% of initial activity | Pseudomonas otitidis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 150 mM, about 130% of initial activity | Pseudomonas otitidis | |
| Na+ | 50 mM, about 115% of initial activity | Pseudomonas otitidis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 205000 | - |
PAGE | Pseudomonas otitidis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas otitidis | A0A1I0U0N3 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 107.84 | - |
pH 8.6, 37°C | Pseudomonas otitidis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Pseudomonas otitidis | L-aspartate + NH3 | - |
? | |
| additional information | poor or no substrates: D-asparagine, L-glutamine, D-glutamine, L-histidine, L-ornithine, Boc-L-asapragine, N-alpha-acetyl-L-asparagine, urea, acrylamide | Pseudomonas otitidis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 34000, SDS-PAGE | Pseudomonas otitidis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SAMN05216263_10744 | - |
Pseudomonas otitidis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pseudomonas otitidis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Pseudomonas otitidis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas otitidis | isoelectric focussing | - |
5.5 |
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