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Literature summary for 3.5.1.1 extracted from

  • Kebeish, R.; El-Sayed, A.; Fahmy, H.; Abdel-Ghany, A.
    Molecular cloning, biochemical characterization, and antitumor properties of a novel L-asparaginase from Synechococcus elongatus PCC6803 (2016), Biochemistry (Moscow), 81, 1173-1181 .
    View publication on PubMed
Search for more literature for 3.5.1.1

Application

Application Comment Organism
medicine enzyme shows antitumor proterties with IC50 values 0.036 mg/ml for MCF-7 cells, 0.037 mM/ml for HCT-116 cells, 0.046 mg/ml for Hep-G2 cells, respectively Synechococcus elongatus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Synechococcus elongatus

Organism

Organism UniProt Comment Textmining
Synechococcus elongatus
-
-
-
Synechococcus elongatus PCC 6803
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant potein Synechococcus elongatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Synechococcus elongatus L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Synechococcus elongatus PCC 6803 L-aspartate + NH3
-
 ?
additional information less than 0.5% of the activity with L-asparagine: L-glutamine, D-asparagine, D-glutamine Synechococcus elongatus ?
-
 ?
additional information less than 0.5% of the activity with L-asparagine: L-glutamine, D-asparagine, D-glutamine Synechococcus elongatus PCC 6803 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Synechococcus elongatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45
-
 about 25% of maximum activity Synechococcus elongatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Synechococcus elongatus

pH Range

pH Minimum pH Maximum Comment Organism
8
-
 about 75% of maximum activity Synechococcus elongatus