Application | Comment | Organism |
---|---|---|
analysis | method based on IR spectroscopy to determine PEG-chitosan copolymer composition as well as composition of copolymer-enzyme conjugates. The method is reagentx02free and allows fast and reliable determination of parameters | Pectobacterium carotovorum |
synthesis | conjugation of enzyme to PEG-chitosan and glycol-chitosan imoproves catalytic efficiency 3- to 6fold under physiological conditions and enhances its resistance to thermal inactivation | Pectobacterium carotovorum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pectobacterium carotovorum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium carotovorum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Pectobacterium carotovorum | L-aspartate + NH3 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
native enzyme, 50 min, complete loss of activity. Enzyme conjugated to glycol-chitosan, 50 min, 40% residual activity | Pectobacterium carotovorum |