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Literature summary for 3.5.1.1 extracted from

  • Huang, L.; Liu, Y.; Sun, Y.; Yan, Q.; Jiang, Z.
    Biochemical characterization of a novel L-asparaginase with low glutaminase activity from Rhizomucor miehei and its application in food safety and leukemia treatment (2014), Appl. Environ. Microbiol., 80, 1561-1569 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
food industry the final level of acrylamide in biscuits and bread is decreased by about 81.6% and 94.2%, respectively, upon treatment with 10 U asnase per mg flour Rhizomucor miehei
medicine enzyme potentiates a lectin's induction of leukemic K562 cell apoptosis, allowing lowering of the drug dosage and shortening of the incubation time Rhizomucor miehei

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhizomucor miehei

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 55% residual activity Rhizomucor miehei
Ag+ 2 mM, 42% residual activity Rhizomucor miehei
Ba2+ 2 mM, 30% residual activity Rhizomucor miehei
Co2+ 2 mM, 69% residual activity Rhizomucor miehei
Cr2+ 2 mM, 37% residual activity Rhizomucor miehei
Cu2+ 2 mM, 13% residual activity Rhizomucor miehei
EDTA 24% residual activity Rhizomucor miehei
Fe3+ 2 mM, 41% residual activity Rhizomucor miehei
Hg2+ 2 mM, 24% residual activity Rhizomucor miehei
Mg2+ 2 mM, 28% residual activity Rhizomucor miehei
SDS 56% residual activity Rhizomucor miehei
Zn2+ 2 mM, 17% residual activity Rhizomucor miehei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.133
-
L-asparagine pH 7.0, 45°C Rhizomucor miehei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
133500
-
gel filtration Rhizomucor miehei

Organism

Organism UniProt Comment Textmining
Rhizomucor miehei W0G253
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1985
-
pH 7.0, 45°C Rhizomucor miehei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
Rhizomucor miehei L-aspartate + NH3
-
?
additional information enzyme shows negligible activity with both D-asparagine (22 U/mg) and L-glutamine (36 U/mg) as the substrates Rhizomucor miehei ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 75316, calculated, 2 * 72000, SDS-PAGE Rhizomucor miehei

Synonyms

Synonyms Comment Organism
ASNase
-
Rhizomucor miehei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Rhizomucor miehei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
stable for 30 min Rhizomucor miehei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.025
-
L-asparagine pH 7.0, 45°C Rhizomucor miehei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Rhizomucor miehei

pH Range

pH Minimum pH Maximum Comment Organism
4 8 more than 80% of maxiumum activity Rhizomucor miehei

pI Value

Organism Comment pI Value Maximum pI Value
Rhizomucor miehei calculated
-
6.8