Application | Comment | Organism |
---|---|---|
food industry | the final level of acrylamide in biscuits and bread is decreased by about 81.6% and 94.2%, respectively, upon treatment with 10 U asnase per mg flour | Rhizomucor miehei |
medicine | enzyme potentiates a lectin's induction of leukemic K562 cell apoptosis, allowing lowering of the drug dosage and shortening of the incubation time | Rhizomucor miehei |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhizomucor miehei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 55% residual activity | Rhizomucor miehei | |
Ag+ | 2 mM, 42% residual activity | Rhizomucor miehei | |
Ba2+ | 2 mM, 30% residual activity | Rhizomucor miehei | |
Co2+ | 2 mM, 69% residual activity | Rhizomucor miehei | |
Cr2+ | 2 mM, 37% residual activity | Rhizomucor miehei | |
Cu2+ | 2 mM, 13% residual activity | Rhizomucor miehei | |
EDTA | 24% residual activity | Rhizomucor miehei | |
Fe3+ | 2 mM, 41% residual activity | Rhizomucor miehei | |
Hg2+ | 2 mM, 24% residual activity | Rhizomucor miehei | |
Mg2+ | 2 mM, 28% residual activity | Rhizomucor miehei | |
SDS | 56% residual activity | Rhizomucor miehei | |
Zn2+ | 2 mM, 17% residual activity | Rhizomucor miehei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.133 | - |
L-asparagine | pH 7.0, 45°C | Rhizomucor miehei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
133500 | - |
gel filtration | Rhizomucor miehei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizomucor miehei | W0G253 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1985 | - |
pH 7.0, 45°C | Rhizomucor miehei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Rhizomucor miehei | L-aspartate + NH3 | - |
? | |
additional information | enzyme shows negligible activity with both D-asparagine (22 U/mg) and L-glutamine (36 U/mg) as the substrates | Rhizomucor miehei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 75316, calculated, 2 * 72000, SDS-PAGE | Rhizomucor miehei |
Synonyms | Comment | Organism |
---|---|---|
ASNase | - |
Rhizomucor miehei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Rhizomucor miehei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable for 30 min | Rhizomucor miehei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
L-asparagine | pH 7.0, 45°C | Rhizomucor miehei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhizomucor miehei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | more than 80% of maxiumum activity | Rhizomucor miehei |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhizomucor miehei | calculated | - |
6.8 |