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Literature summary for 3.5.1.1 extracted from

  • Han, S.; Jung, J.; Park, W.
    Biochemical characterization of L-asparaginase in NaCl-tolerant Staphylococcus sp. OJ82 isolated from fermented seafood (2014), J. Microbiol. Biotechnol., 24, 1096-1104.
    View publication on PubMed
Search for more literature for 3.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus sp.

General Stability

General Stability Organism
the enzyme retains more than 60% of its activity under 2 M NaCl condition Staphylococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ about 25% residual activity at 10 mM Staphylococcus sp.
Ni2+ about 90% residual activity at 10 mM Staphylococcus sp.
Zn2+ about 90% residual activity at 10 mM Staphylococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
 L-asparagine at pH 8.6 and 37°C Staphylococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ about 263.9% activity at 10 mM Staphylococcus sp.
Cu2+ about 109.3% activity at 10 mM Staphylococcus sp.
Fe2+ about 203.7% activity at 10 mM Staphylococcus sp.
additional information not influenced by K+ Staphylococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37500
-
 2 * 37500, SDS-PAGE Staphylococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Staphylococcus sp. 100% activity L-aspartate + NH3
-
 ?
L-asparagine + H2O Staphylococcus sp. OJ82 100% activity L-aspartate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus sp. J9H7D1
-
-
Staphylococcus sp. OJ82 J9H7D1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Staphylococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.88
-
 crude cell extract, at pH 8.6 and 37°C Staphylococcus sp.
113.1
-
 after 128.47fold purification, at pH 8.6 and 37°C Staphylococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O 100% activity Staphylococcus sp. L-aspartate + NH3
-
 ?
L-asparagine + H2O 100% activity Staphylococcus sp. OJ82 L-aspartate + NH3
-
 ?
L-glutamine + H2O 58.2% activity compared to L-asparagine Staphylococcus sp. ?
-
 ?
L-glutamine + H2O 58.2% activity compared to L-asparagine Staphylococcus sp. OJ82 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 37500, SDS-PAGE Staphylococcus sp.

Synonyms

Synonyms Comment Organism
Asn
-
 Staphylococcus sp.
L-asparaginase
-
 Staphylococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Staphylococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 50 over 90% of activity is retained between 37°C and 50°C. Below 25°C, the enzyme loses most of its activity Staphylococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 50 the enzyme shows no loss of activity after 30 min at 37°C and 40% loss of activity after 30 min at 50°C Staphylococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.65
-
 L-asparagine at pH 8.6 and 37°C Staphylococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Staphylococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
8 11 more than 50% activity between pH 8.0 and 11.0, while activity is dramatically decreased to 20% below pH 8.0 Staphylococcus sp.