Literature summary for 3.5.1.1 extracted from

Hong, S.J.; Lee, Y.H.; Khan, A.R.; Ullah, I.; Lee, C.; Park, C.K.; Shin, J.H.
Cloning, expression, and characterization of thermophilic L-asparaginase from Thermococcus kodakarensis KOD1 (2014), J. Basic Microbiol., 54, 500-508.

Search for more literature for 3.5.1.1

Application

Application	Comment	Organism
food industry	the acrylamide contents in baked dough were reduced to sixty percent after treatment with recombinant enzyme as compared to the untreated control	Thermococcus kodakarensis

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BLR(DE3) cells	Thermococcus kodakarensis

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	80% residual activity at 1 mM	Thermococcus kodakarensis
Co2+	complete inhibition at 1 mM	Thermococcus kodakarensis
Cu2+	15% residual activity at 1 mM	Thermococcus kodakarensis
EDTA	90% residual activity at 1 mM	Thermococcus kodakarensis
Ni2+	complete inhibition at 1 mM	Thermococcus kodakarensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.6	-	L-asparagine	at pH 8.0 and 90°C	Thermococcus kodakarensis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	138% activity at 1 mM	Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	2 * 37000, SDS-PAGE	Thermococcus kodakarensis
71000	-	gel filtration	Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-asparagine + H2O	Thermococcus kodakarensis	-	L-aspartate + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermococcus kodakarensis	Q5JHT1	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, and gel filtration	Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
541.9	-	crude enzyme, at pH 8.0 and 90°C	Thermococcus kodakarensis
978.7	-	after 1.8fold purification, at pH 8.0 and 90°C	Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-asparagine + H2O	-	Thermococcus kodakarensis	L-aspartate + NH3	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 37000, SDS-PAGE	Thermococcus kodakarensis

Synonyms

Synonyms	Comment	Organism
Asn	-	Thermococcus kodakarensis
L-asparaginase	-	Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
90	-	-	Thermococcus kodakarensis

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
70	100	the enzyme retains more than 50% activity between 70 and 100°C	Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
90	-	the enzyme retains almost 90% of its activity after 32 h incubation at 90°C	Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
694	-	L-asparagine	at pH 8.0 and 90°C	Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Thermococcus kodakarensis

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	10	the enzyme retains 20-90% of its activity between pH 6.0 and 10.0	Thermococcus kodakarensis

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Release 2023.1 (January 2023)