Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycine | glycine stimulates self-cleavage in a dose-dependent manner | Cavia porcellus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Cavia porcellus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M MES at pH 6.0, and 13-15% (w/v) PEG 3350 | Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
beta-L-Asp-L-Phe | at pH 7.5 and 37°C | Cavia porcellus | |
2.24 | - |
L-asparagine | at pH 7.5 and 37°C | Cavia porcellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
1 * 20000 + 1 * 15000, active, cleaved enzyme | Cavia porcellus |
20000 | - |
1 * 20000 + 1 * 15000, active, cleaved enzyme | Cavia porcellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Cavia porcellus | - |
L-aspartate + NH3 | - |
? | |
additional information | Cavia porcellus | the enzyme requires autocleavage to become active | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap HP Ni Sepharose column chromatography and Superdex 200 gel filtration | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-L-Asp-L-Phe + H2O | - |
Cavia porcellus | ? | - |
? | |
L-asparagine + H2O | - |
Cavia porcellus | L-aspartate + NH3 | - |
? | |
additional information | the enzyme requires autocleavage to become active | Cavia porcellus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 20000 + 1 * 15000, active, cleaved enzyme | Cavia porcellus |
Synonyms | Comment | Organism |
---|---|---|
ASNase3 | - |
Cavia porcellus |
L-asparaginase type III | - |
Cavia porcellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.98 | - |
beta-L-Asp-L-Phe | at pH 7.5 and 37°C | Cavia porcellus | |
3.95 | - |
L-asparagine | at pH 7.5 and 37°C | Cavia porcellus |
General Information | Comment | Organism |
---|---|---|
additional information | ASNase3 is not the enzyme responsible for the antitumor effects of guinea pig serum | Cavia porcellus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.76 | - |
L-asparagine | at pH 7.5 and 37°C | Cavia porcellus | |
4.83 | - |
beta-L-Asp-L-Phe | at pH 7.5 and 37°C | Cavia porcellus |