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Literature summary for 3.5.1.1 extracted from
- Recombinant deamidated mutants of Erwinia chrysanthemi L-asparaginase have similar or increased activity compared to wild-type enzyme (2014), Mol. Biotechnol., 56, 865-877.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzyme is an important biopharmaceutical product used in the treatment of acute lymphoblastic leukaemia | Dickeya chrysanthemi |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutants containing Asn to Asp changes | Dickeya chrysanthemi |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N281D | mutant has approximately the same specific activity as the wild-type enzyme. Mutation results in a lower glutaminase activity compared with wild-type and the N41D mutant. Mutation imparts less stability to the enzyme at elevated temperatures. The N281D mutation causes a decrease in substrate affinity as well as a decrease in the stability profile. The deamidation at the N281 site should not be a concern during processing, storage or clinical use. The deamidated variant is active and stable under normal storage conditions | Dickeya chrysanthemi |
| N41D | mutant has approximately the same specific activity as the wild-type enzyme. Mutation conferrs a slight increase in kcat. Charge differences to the wild-type enzyme, at -1 per monomer or -4 per tetramer. The deamidation at the N41 site should not be a concern during processing, storage or clinical use. These deamidated variant is active and stable under normal storage conditions | Dickeya chrysanthemi |
| N41D/N281D | mutant enzyme has increased specific activity. Charge differences to the wild-type enzyme, at -1 per monomer or -4 per tetramer. The N281D mutation causes a decrease in substrate affinity as well as a decrease in the stability profile. The deamidation at the N41 and N281 sites should not be a concern during processing, storage or clinical use. These deamidated variant is active and stable under normal storage conditions | Dickeya chrysanthemi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0291 | - |
L-asparagine | pH 8.6, 37°C, mutant enzyme N41D | Dickeya chrysanthemi |  |
| 0.0291 | - |
L-asparagine | pH 8.6, 37°C, wild-type enzyme | Dickeya chrysanthemi | |
| 0.0562 | - |
L-asparagine | pH 8.6, 37°C, mutant enzyme N41D/N281D | Dickeya chrysanthemi | |
| 0.0597 | - |
L-asparagine | pH 8.6, 37°C, mutant enzyme N281D | Dickeya chrysanthemi | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| urea | the N281D mutation results in an inability to refold after exposure to urea concentrations greater than 1.5 M, while the external N41D mutation has no impact on the stability of the enzyme | Dickeya chrysanthemi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dickeya chrysanthemi | - |
- |
- |
| Dickeya chrysanthemi NCPPB 1066 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mutants containing Asn to Asp changes | Dickeya chrysanthemi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Dickeya chrysanthemi | L-aspartate + NH3 | - |
? | |
| L-asparagine + H2O | - |
Dickeya chrysanthemi NCPPB 1066 | L-aspartate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | - |
Dickeya chrysanthemi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ErA | - |
Dickeya chrysanthemi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Dickeya chrysanthemi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 49 | - |
3 min, 50% loss of activity of mutant enzyme N281D and double mutant enzyme N41D/N281D, no loss of activity of wild-type enzyme and mutant enzyme N41D | Dickeya chrysanthemi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 565 | - |
L-asparagine | pH 8.6, 37°C, wild-type enzyme | Dickeya chrysanthemi | |
| 573 | - |
L-asparagine | pH 8.6, 37°C, mutant enzyme N281D | Dickeya chrysanthemi | |
| 657 | - |
L-asparagine | pH 8.6, 37°C, mutant enzyme N41D | Dickeya chrysanthemi | |
| 798 | - |
L-asparagine | pH 8.6, 37°C, mutant enzyme N41D/N281D | Dickeya chrysanthemi | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
assay at | Dickeya chrysanthemi |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Dickeya chrysanthemi | wild-type and N281D enzymes have roughly the same pI, as do the N41D and the double mutant | - |
additional information |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9597 | - |
L-asparagine | pH 8.6, 37°C, mutant enzyme N281D | Dickeya chrysanthemi | |
| 14200 | - |
L-asparagine | pH 8.6, 37°C, mutant enzyme N41D/N281D | Dickeya chrysanthemi | |
| 19420 | - |
L-asparagine | pH 8.6, 37°C, wild-type enzyme | Dickeya chrysanthemi | |
| 22580 | - |
L-asparagine | pH 8.6, 37°C, mutant enzyme N41D | Dickeya chrysanthemi | |
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