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Literature summary for 3.5.1.1 extracted from

  • Moreno-Enriquez, A.; Evangelista-Martinez, Z.; Gonzalez-Mondragon, E.G.; Calderon-Flores, A.; Arreguin, R.; Perez-Rueda, E.; Huerta-Saquero, A.
    Biochemical characterization of recombinant L-asparaginase (AnsA) from Rhizobium etli, a member of an increasing rhizobial-type family of L-asparaginases (2012), J. Microbiol. Biotechnol., 22, 292-300.
    View publication on PubMed
Search for more literature for 3.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene ansA, phylogenetic analysis, expression of His-tagged AnsA in Escherichia coli strain BL21(DE3) Rhizobium etli

Inhibitors

Inhibitors Comment Organism Structure
aspartic acid competitive Rhizobium etli
beta-aspartyl hydroxamate 48% inhibition at 5 mM Rhizobium etli
Ca2+ 31% inhibition at 1 mM Rhizobium etli
D-asparagine 39% inhibition at 5 mM Rhizobium etli
Mg2+ 31% inhibition at 1 mM Rhizobium etli
Mn2+ 52% inhibition at 1 mM Rhizobium etli
additional information poor inhibition by L-glutamine at 5 mM Rhizobium etli
NH4+ noncompetitive Rhizobium etli
Zn2+ 38% inhibition at 1 mM Rhizobium etli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.9
-
 L-asparagine pH 7.5, 37°C, recombinant enzyme Rhizobium etli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 47000, recombinant His-tagged enzyme, SDS-PAGE Rhizobium etli
102000
-
 recombinant His-tagged enzyme, native PAGE Rhizobium etli

Organism

Organism UniProt Comment Textmining
Rhizobium etli Q9RFN5 gene ansA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged AnsA 18fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity Rhizobium etli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
 purified recombinant enzyme, pH 7.5, 37°C Rhizobium etli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Rhizobium etli ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 47000, recombinant His-tagged enzyme, SDS-PAGE Rhizobium etli

Synonyms

Synonyms Comment Organism
AnsA
-
 Rhizobium etli
L-asparaginase
-
 Rhizobium etli
L-asparaginase II
-
 Rhizobium etli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rhizobium etli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 activity range, temperature profile overview Rhizobium etli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified recombinnat enzyme, loss of 60% activity after 20 min, inactivation within 30 min Rhizobium etli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
106
-
 L-asparagine pH 7.5, 37°C, recombinant enzyme Rhizobium etli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Rhizobium etli

pH Range

pH Minimum pH Maximum Comment Organism
7 11 activity range, pH profile overview Rhizobium etli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
10
-
 NH4+ pH 7.5, 37°C, recombinant enzyme Rhizobium etli
33
-
 aspartic acid pH 7.5, 37°C, recombinant enzyme Rhizobium etli

General Information

General Information Comment Organism
evolution the enzyme is a member of an increasing rhizobial-type family of L-asparaginases Rhizobium etli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12
-
 L-asparagine pH 7.5, 37°C, recombinant enzyme Rhizobium etli