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Literature summary for 3.5.1.1 extracted from

  • Sidoruk, K.; Pokrovsky, V.; Borisova, A.; Omeljanuk, N.; Aleksandrova, S.; Pokrovskaya, M.; Gladilina, J.; Bogush, V.; Sokolov, N.
    Creation of a producent, optimization of expression, and purifi cation of recombinant Yersinia Pseudotuberculosis L-asparaginase (2011), Bull. Exp. Biol. Med., 152, 219-223.
    View publication on PubMed
Search for more literature for 3.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene ansB, expression in Escherichia coli strain BL21 (DE3), method evaluation, overview Yersinia pseudotuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Yersinia pseudotuberculosis
-
 L-aspartate + NH3
-
 ?
L-asparagine + H2O Yersinia pseudotuberculosis IP32953
-
 L-aspartate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Yersinia pseudotuberculosis Q66CJ2 gene ansB
-
Yersinia pseudotuberculosis IP32953 Q66CJ2 gene ansB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant YpA from Escherichia coli strain BL21 (DE3) by two different steps of anion exchange chromatography Yersinia pseudotuberculosis

Source Tissue

Source Tissue Comment Organism Textmining
cell culture optimal conditions of induction are: inductor concentration of 0.1-0.2%, its addition during the middle of the logphase at 1.45-2.3 OU600, and culturing temperature of 28°C. The optimal duration of culturing after induction is 7-16 h Yersinia pseudotuberculosis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
62.7
-
 pH 8.0, 37°C, purified enzyme Yersinia pseudotuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Yersinia pseudotuberculosis L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Yersinia pseudotuberculosis IP32953 L-aspartate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
L-asparaginase II
-
 Yersinia pseudotuberculosis
Ypa
-
 Yersinia pseudotuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Yersinia pseudotuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Yersinia pseudotuberculosis

Expression

Organism Comment Expression
Yersinia pseudotuberculosis optimal conditions of induction are: inductor concentration of 0.1-0.2%, its addition during the middle of the logphase at 1.45-2.3 OU600, and culturing temperature of 28°C. The optimal duration of culturing after induction is 7-16 h up