KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
L-asparagine | pH 8.6, 37°C | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibits enzyme, 1, 3, and 10 mM tested | Streptomyces sp. | |
EDTA | inhibits enzyme, 1, 3, and 10 mM tested | Streptomyces sp. | |
Mn2+ | activates enzyme, 1, 3, and 10 mM tested | Streptomyces sp. | |
Zn2+ | inhibits enzyme, 1, 3, and 10 mM tested | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
marine actinomycetes, soil isolate S1, S2, S3, S4, S5, S6, S8. K2, K4, K5, and K8, whereof S3, S4 and S6 are designated as Streptomyces spp. | - |
Purification (Comment) | Organism |
---|---|
to homogeneity by ammonium sulfate precipitation, dialysis, gel filtration on Sephadex G-100 and SDS-PAGE | Streptomyces sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
soil | sediment samples obtained from Tamilnadu and Kerala in India, production of the enzyme in three different media, solid-State media, tryptone glucose yeast extract, and tryptone fructose yeast extract broth. 10 isolates (S1, S2, S3, S4, S5, S6, S8. K2, K4, K5, and K8), only S3, S4 and K8 show L-asparaginase activity | Streptomyces sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
662.6 | - |
purified enzyme, 37°C, pH 8.6 | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Streptomyces sp. | L-aspartate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-asparaginase | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces sp. |
50 | - |
- |
Streptomyces sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 75 | ca. 30 U/ml at 30°C and 75°C | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
maximum activity between pH 7.0 and 8.0 with 80% activity at physiological pH | Streptomyces sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | 20 U/ml enzyme activity at pH 5 and 10 | Streptomyces sp. |
8.6 | - |
assay at | Streptomyces sp. |