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Literature summary for 3.5.1.1 extracted from

  • Mohamed Ali, E.
    Purification and characterization of Vigna unguiculata cultivar asparaginase (2009), J. Biol. Res., 11, 29-36.
No PubMed abstract available
Search for more literature for 3.5.1.1

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 5 mM, 37°C, 25% relative activity compared to the activity in absence of metal cations Vigna unguiculata
Hg2+ 5 mM, 37°C, 20% relative activity compared to the activity in absence of metal cations Vigna unguiculata
Mn2+ 5 mM, 37°C, 42% relative activity compared to the activity in absence of metal cations Vigna unguiculata
Zn2+ 5 mM, 37°C, 36% relative activity compared to the activity in absence of metal cations Vigna unguiculata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.25
-
 L-asparagine pH 8.0, 37°C Vigna unguiculata

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 5 mM, 37°C, 134% relative activity compared to the activity in absence of metal cations Vigna unguiculata
Ni2+ 5 mM, 37°C, 136% relative activity compared to the activity in absence of metal cations Vigna unguiculata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE, indicates this enzyme has two similar subunits Vigna unguiculata
70000
-
 gel filtration Vigna unguiculata

Organism

Organism UniProt Comment Textmining
Vigna unguiculata
-
 various cultivars: Kareem 7, Dokki 331, Kafer El-Sheikh 1, Kaha 1, and Fodder
-

Purification (Commentary)

Purification (Comment) Organism
all three forms ASPG I, ASPG II, and ASPG II are purified, ASPG II shows highest specific activity, purity 9.27% and 36% recovery Vigna unguiculata

Source Tissue

Source Tissue Comment Organism Textmining
seed germinating and non-germinating seeds are studied, highest specific activity in germinating seeds in all cultivars Vigna unguiculata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.3
-
 cultivar Dokki 331, non-germinating seeds, pH 8.0, 37°C Vigna unguiculata
16.8
-
 cultivar Kaha 1, non-germinating seeds, pH 8.0, 37°C Vigna unguiculata
18.5
-
 cultivar Kareem 7, non-germinating seeds, pH 8.0, 37°C Vigna unguiculata
24.3
-
 cultivar Kafer El-Sheikh 1, non-germinating seeds, pH 8.0, 37°C Vigna unguiculata
32.3
-
 cultivar Fodder, non-germinating seeds, pH 8.0, 37°C Vigna unguiculata
49
-
 cultivar Dokki 331, germinating seeds, pH 8.0, 37°C Vigna unguiculata
63
-
 cultivar Kaha 1, germinating seeds, pH 8.0, 37°C Vigna unguiculata
74
-
 cultivar Kareem 7, germinating seeds, pH 8.0, 37°C Vigna unguiculata
87
-
 cultivar Kafer El-Sheikh 1, germinating seeds, pH 8.0, 37°C Vigna unguiculata
105
-
 cultivar Fodder, non-germinating seeds, pH 8.0, 37°C Vigna unguiculata
969
-
 ASPG II, from germinating seeds, cultivar Fodder, pH 8.0, 37°C Vigna unguiculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Vigna unguiculata L-aspartate + NH3
-
 ?
L-aspartyl hydroxamate + H2O 60% relative activity compared to L-asparagine as substrate Vigna unguiculata L-aspartate + hydroxylamine
-
 ?
L-glutamine + H2O 36% relative activity compared to L-asparagine as substrate Vigna unguiculata L-glutamate + NH3
-
 ?
L-glutamyl hydroxamate + H2O 16% relative activity compared to L-asparagine as substrate Vigna unguiculata L-glutamate + hydroxylamine
-
 ?
additional information no activity with D-asparagine as substrate Vigna unguiculata ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE, indicates this enzyme has two similar subunits Vigna unguiculata

Synonyms

Synonyms Comment Organism
ASPG II three forms, ASPG I, ASPG II, and ASPG III. ASPG II shows the highest specific activity Vigna unguiculata
L-asparaginase
-
 Vigna unguiculata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Vigna unguiculata
40
-
-
 Vigna unguiculata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 20% relative activity at 10 and 60°C Vigna unguiculata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up to 40°C followed by sharp decline Vigna unguiculata
90
-
 loses about 95% activity at 90°C Vigna unguiculata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 phosphate buffer Vigna unguiculata

pH Range

pH Minimum pH Maximum Comment Organism
5 9 20% relative activity at pH 5 and 40% relative activity at pH 9 Vigna unguiculata