Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.1 extracted from

  • Abakumova, O.; Podobed, O.; Borisova, A.; Sidoruk, K.; Alexandrova, S.; Omelyanuk, N.; Pokrovskaya, M.; Kondakova, L.; Sokolov, N.
    Antitumor activity of L-asparaginase from Yersinia pseudotuberculosis (2009), Biochemistry (Moscow) Suppl. Ser. B, 3, 198-201.
No PubMed abstract available
Search for more literature for 3.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
cloned and expressed in Escherichia coli BL21 (DE3) cells Yersinia pseudotuberculosis

Organism

Organism UniProt Comment Textmining
Pectobacterium carotovorum
-
-
-
Yersinia pseudotuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
56% yield, purity is more than 95% Yersinia pseudotuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
106
-
 purified protein, 37°C, pH not specified in this publication Yersinia pseudotuberculosis

Synonyms

Synonyms Comment Organism
L-asparaginase
-
 Yersinia pseudotuberculosis
L-asparaginase
-
 Pectobacterium carotovorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Yersinia pseudotuberculosis
37
-
 assay at Pectobacterium carotovorum

pI Value

Organism Comment pI Value Maximum pI Value
Yersinia pseudotuberculosis isoelectrofocusing
-
 5.4

General Information

General Information Comment Organism
physiological function the cytotoxic activity of L-asparaginase from Yersinia pseudotuberculosis and Erwinia carotovora are investigated and compared in vitro using human T-lymphoblastic leukemia (Jurkat and Molt-4 cells) and also solid tumor cell lines MCF-7 (human breast adenocarcinoma), LnCap (human prostate carcinoma), NGUK1 (ratGasser node neurinoma). The data obtained indicate that the Yersinia pseudotuberculosis L-asparaginase significantly inhibits growth of leukemic and solid tumor cells. Comparing effectiveness of L-asparaginases from various sources one can see that L-asparaginase from Yersinia pseudotuberculosis exhibits higher activity in suppression of tumor cell growth than the enzyme from Erwinia carotovora Yersinia pseudotuberculosis
physiological function the cytotoxic activity of L-asparaginase from Yersinia pseudotuberculosis and Erwinia carotovora are investigated and compared in vitro using human T-lymphoblastic leukemia (Jurkat and Molt-4 cells) and also solid tumor cell lines MCF-7 (human breast adenocarcinoma), LnCap (human prostate carcinoma), NGUK1 (ratGasser node neurinoma). The data obtained indicate that the Yersinia pseudotuberculosis L-asparaginase significantly inhibits growth of leukemic and solid tumor cells. Comparing effectiveness of L-asparaginases from various sources one can see that L-asparaginase from Yersinia pseudotuberculosis exhibits higher activity in suppression of tumor cell growth than the enzyme from Erwinia carotovora Pectobacterium carotovorum