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Literature summary for 3.5.1.1 extracted from
- Withania somnifera (Ashwagandha): a novel source of L-asparaginase (2009), J. Integr. Plant Biol., 51, 201-206.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.061 | - |
L-asparagine | - |
Withania somnifera |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
2 * 36000, SDS-PAGE, gel filtration | Withania somnifera |
| 72000 | - |
gel filtration, PAGE | Withania somnifera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Capsicum annuum | - |
- |
- |
| Datura inoxia | - |
- |
- |
| Pisum sativum | - |
- |
- |
| Solanum lycopersicum | - |
- |
- |
| Tamarindus indica | - |
- |
- |
| Vigna unguiculata | - |
subsp. unguiculata | - |
| Withania somnifera | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Withania somnifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Pisum sativum | - |
| fruit | - |
Datura inoxia | - |
| fruit | - |
Capsicum annuum | - |
| leaf | - |
Solanum lycopersicum | - |
| leaf | - |
Tamarindus indica | - |
| leaf | - |
Vigna unguiculata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1893 | - |
- |
Withania somnifera |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Pisum sativum | L-aspartic acid + NH3 | - |
? | |
| L-asparagine + H2O | - |
Datura inoxia | L-aspartic acid + NH3 | - |
? | |
| L-asparagine + H2O | - |
Solanum lycopersicum | L-aspartic acid + NH3 | - |
? | |
| L-asparagine + H2O | - |
Tamarindus indica | L-aspartic acid + NH3 | - |
? | |
| L-asparagine + H2O | - |
Capsicum annuum | L-aspartic acid + NH3 | - |
? | |
| L-asparagine + H2O | - |
Vigna unguiculata | L-aspartic acid + NH3 | - |
? | |
| L-asparagine + H2O | - |
Withania somnifera | L-aspartic acid + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 36000, SDS-PAGE, gel filtration | Withania somnifera |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| asparaginase | - |
Pisum sativum |
| asparaginase | - |
Datura inoxia |
| asparaginase | - |
Solanum lycopersicum |
| asparaginase | - |
Tamarindus indica |
| asparaginase | - |
Capsicum annuum |
| asparaginase | - |
Vigna unguiculata |
| asparaginase | - |
Withania somnifera |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Withania somnifera |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 178.6 | - |
L-asparagine | - |
Withania somnifera | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Withania somnifera |
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Release 2023.1 (January 2023)
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