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Literature summary for 3.5.1.1 extracted from

  • Kotzia, G.A.; Labrou, N.E.
    Engineering thermal stability of L-asparaginase by in vitro directed evolution (2009), FEBS J., 276, 1750-1761.
    View publication on PubMed
Search for more literature for 3.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pectobacterium carotovorum
-
 Dickeya chrysanthemi

Protein Variants

Protein Variants Comment Organism
D133I analysis of the contribution of this position on thermostability Dickeya chrysanthemi
D133L analysis of the contribution of this position on thermostability Dickeya chrysanthemi
D133T analysis of the contribution of this position on thermostability Dickeya chrysanthemi
D133V analysis of the contribution of this position on thermostability Dickeya chrysanthemi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 L-asparagine mutant D133T Dickeya chrysanthemi
0.097
-
 L-asparagine mutant D133I Dickeya chrysanthemi
0.153
-
 L-asparagine mutant D133V Dickeya chrysanthemi
0.16
-
 L-asparagine mutant D133L Dickeya chrysanthemi
0.597
-
 N-acetyl-L-asparagine mutant D133V Dickeya chrysanthemi
1.008
-
 L-glutamine mutant D133T Dickeya chrysanthemi
1.099
-
 L-glutamine mutant D133I Dickeya chrysanthemi
1.677
-
 L-glutamine mutant D133L Dickeya chrysanthemi
1.788
-
 N-acetyl-L-asparagine mutant D133L Dickeya chrysanthemi
1.999
-
 L-glutamine mutant D133V Dickeya chrysanthemi
2.096
-
 N-acetyl-L-asparagine mutant D133T Dickeya chrysanthemi
5.613
-
 N-acetyl-L-asparagine mutant D133I Dickeya chrysanthemi

Organism

Organism UniProt Comment Textmining
Dickeya chrysanthemi Q6Q4F3
-
-
Pectobacterium carotovorum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pectobacterium carotovorum
-
 Dickeya chrysanthemi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Pectobacterium carotovorum L-aspartic acid + NH3
-
 ?
L-asparagine + H2O
-
 Dickeya chrysanthemi L-aspartic acid + NH3
-
 ?
L-glutamine + H2O
-
 Pectobacterium carotovorum L-glutamic acid + NH3
-
 ?
L-glutamine + H2O
-
 Dickeya chrysanthemi L-glutamic acid + NH3
-
 ?
N-acetyl-L-asparagine + H2O
-
 Dickeya chrysanthemi N-acetyl-L-aspartic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
asparaginase
-
 Pectobacterium carotovorum
asparaginase
-
 Dickeya chrysanthemi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
46.4
-
 wild type, half-inactivation temperature, 7.5 min Dickeya chrysanthemi
50
-
 mutant D133V, half-inactivation after 159.7 h Dickeya chrysanthemi
50
-
 wild type, half-inactivation after 2.7 h Dickeya chrysanthemi
55
-
 mutant D133V, after 7.5 min 87.3% remaining activity Dickeya chrysanthemi
55
-
 wild type, after 7.5 min 19.9% remaining activity Pectobacterium carotovorum
55
-
 wild type, after 7.5 min 37.2% remaining activity Dickeya chrysanthemi
55.8
-
 mutant D133V, half-inactivation temperature, 7.5 min Dickeya chrysanthemi