Cloned (Comment) | Organism |
---|---|
gene PH0066, expression in Escherichia coli strain BL21 | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, 0.002 ml or protein solution is mixed with 0.001 ml reservoir solution containing 0.1 M Tris-HCl, pH 8.5, 12% v/v PEG 400, and 30 mM NaCl, soaking of crystals in 25% v/v PEG 400 and flash-cooling, X-ray difraction structure determination and analysis at 2.16 A resolution | Pyrococcus horikoshii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Pyrococcus horikoshii | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Pyrococcus horikoshii | - |
L-aspartate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PhA from Escherichia coli strain BL21 by anion exchange chromatography and gel filtration, followed by hydrophobic interaction chromatography and ultrafiltration | Pyrococcus horikoshii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-asparagine + H2O = L-aspartate + NH3 | active site structure with a flexible loop that is stabilized by a beta-hairpin formation and intracellular interaction with the alpha-helical region, modelling | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Pyrococcus horikoshii | L-aspartate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimer, crystal structure | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
L-asparagine aminohydrolase | - |
Pyrococcus horikoshii |
PhA | - |
Pyrococcus horikoshii |
type I L-asparaginase | - |
Pyrococcus horikoshii |