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Literature summary for 3.5.1.1 extracted from

  • Mohapatra, B.R.; Bajpuji, M.; Banerjee, U.C.
    Production and properties of L-asparaginase from Mucor species associated with a marine sponge (Spirastrella sp.) (1997), Cytobios, 92, 165-173.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 25 mM, 26% loss of activity Mucor sp.
Cu2+ 20 mM, 44% inhibition Mucor sp.
EDTA strong Mucor sp.
Mn2+ 20 mM, 50% inhibition Mucor sp.
NaCl above 4% Mucor sp.
Zn2+ 25 mM, 25% inhibition Mucor sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.7
-
L-Asn
-
Mucor sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Mucor sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ maximal activity at 2% NaCl Mucor sp.

Organism

Organism UniProt Comment Textmining
Mucor sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Mucor sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Asn + H2O
-
Mucor sp. L-Asp + NH3
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Mucor sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life: 60 min Mucor sp.
60
-
half-life: 15 min Mucor sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Mucor sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 8 pH 4.0: about 40% of maximal activity, pH 8.0: about 30% of maximal activity Mucor sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
20
-
Mn2+
-
Mucor sp.