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Literature summary for 3.5.1.1 extracted from
- Purification and some biological properties of asparaginase from Azotobacter vinelandii (1977), Appl. Environ. Microbiol., 33, 508-514.
General Stability
| General Stability | Organism |
|---|---|
| enzyme is stable when incubated with rat serum and ascites fluid | Azotobacter vinelandii |
| stable during lyophilization and concentration with aquacide, inactivation after dialysis | Azotobacter vinelandii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| atabrin | - |
Azotobacter vinelandii |  |
| Cu2+ | - |
Azotobacter vinelandii | |
| Fe2+ | - |
Azotobacter vinelandii | |
| Hg2+ | - |
Azotobacter vinelandii | |
| hydrazine | - |
Azotobacter vinelandii | |
| hydroxylamine | - |
Azotobacter vinelandii | |
| iodoacetate | - |
Azotobacter vinelandii | |
| KCN | - |
Azotobacter vinelandii | |
| p-hydroxymercuribenzoate | - |
Azotobacter vinelandii | |
| potassium ferricyanide | - |
Azotobacter vinelandii | |
| Zn2+ | - |
Azotobacter vinelandii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
L-asparagine | - |
Azotobacter vinelandii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
- |
Azotobacter vinelandii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azotobacter vinelandii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Azotobacter vinelandii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.47 | - |
- |
Azotobacter vinelandii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 20 days | Azotobacter vinelandii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Asn + H2O | - |
Azotobacter vinelandii | L-Asp + NH3 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48 | - |
- |
Azotobacter vinelandii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable up to | Azotobacter vinelandii |
| 65 | - |
inactivation at | Azotobacter vinelandii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Azotobacter vinelandii |
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