Activating Compound | Comment | Organism | Structure |
---|---|---|---|
PCMB | slight stimulation | Lupinus polyphyllus | |
Tartrate | stimulation | Lupinus polyphyllus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-5-chloro-4-oxo pentanoic acid | - |
Lupinus polyphyllus | |
3-Cyano-L-Ala | - |
Lupinus polyphyllus | |
4-methylene-L-glutamine | - |
Lupinus polyphyllus | |
5-Bromo-4-oxo-L-norvaline | - |
Lupinus polyphyllus | |
Br- | - |
Lupinus polyphyllus | |
Cd2+ | - |
Lupinus polyphyllus | |
Co2+ | - |
Lupinus polyphyllus | |
Cu2+ | - |
Lupinus polyphyllus | |
Dichromate | - |
Lupinus polyphyllus | |
DL-aspartate 3-hydroxamate | - |
Lupinus polyphyllus | |
Fe2+ | - |
Lupinus polyphyllus | |
Mg2+ | - |
Lupinus polyphyllus | |
N4-hydroxyethyl-L-asparagine | - |
Lupinus polyphyllus | |
Ni2+ | - |
Lupinus polyphyllus | |
Pb4+ | - |
Lupinus polyphyllus | |
Sn2+ | - |
Lupinus polyphyllus | |
sulfate | - |
Lupinus polyphyllus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.2 | - |
L-asparagine | - |
Lupinus polyphyllus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight stimulation | Lupinus polyphyllus | |
diphosphate | stimulation | Lupinus polyphyllus | |
HPO42- | stimulation | Lupinus polyphyllus | |
K3Fe(CN)6 | stimulation | Lupinus polyphyllus | |
Na+ | slight stimulation | Lupinus polyphyllus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE | Lupinus polyphyllus |
72000 | - |
sucrose density gradient centrifugation | Lupinus polyphyllus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lupinus polyphyllus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lupinus polyphyllus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Lupinus polyphyllus | - |
Storage Stability | Organism |
---|---|
-20°C, less than 10% loss of activity after 3 months | Lupinus polyphyllus |
4°C, stable for up to 4 days | Lupinus polyphyllus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-aspartyl hydroxamate + H2O | - |
Lupinus polyphyllus | DL-Asp + hydroxylamine | - |
? | |
L-Asn + H2O | - |
Lupinus polyphyllus | L-Asp + NH3 | - |
? | |
L-Gln + H2O | no activity | Lupinus polyphyllus | L-Glu + NH3 | - |
? | |
N4-ethyl-L-asparagine | 12% of the activity with L-Asn | Lupinus polyphyllus | L-Asp + propylamine | - |
? | |
N4-methoxy-L-asparagine + H2O | 154% of the activity with L-Asn | Lupinus polyphyllus | L-Asp + O-methylhydroxylamine | - |
? | |
N4-methyl-L-asparagine | 12% of the activity with L-Asn | Lupinus polyphyllus | L-Asp + methylamine | - |
? | |
threo-3-hydroxy-L-asparagine + H2O | 36% of the activity with L-Asn | Lupinus polyphyllus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Lupinus polyphyllus |