Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.99.B1 extracted from

  • Plummer, L.J.; Hildebrandt, E.R.; Porter, S.B.; Rogers, V.A.; McCracken, J.; Schmidt, W.K.
    Mutational analysis of the ras converting enzyme reveals a requirement for glutamate and histidine residues (2006), J. Biol. Chem., 281, 4596-4605.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.99.B1

Protein Variants

Protein Variants Comment Organism
C251A wild-type in function Saccharomyces cerevisiae
E156A activity only when overexpressed Saccharomyces cerevisiae
E156D marginal activity, overexpression significantly boosts the activity Saccharomyces cerevisiae
E156Q no activity Saccharomyces cerevisiae
E157A diminished but significant activity Saccharomyces cerevisiae
F190A diminished but significant activity Saccharomyces cerevisiae
H194A invariably inactive Saccharomyces cerevisiae
H196A wild-type in function Saccharomyces cerevisiae
H197A wild-type in function Saccharomyces cerevisiae
H248A activity only when overexpressed Saccharomyces cerevisiae
additional information strain yWS163, that lacks the a-factor encoding genes and the CAAX protease genes Saccharomyces cerevisiae
N252A diminished but significant activity Saccharomyces cerevisiae
Y160A diminished but significant activity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00144
-
 a-factor derived synthetic farnesylated pentadecapeptide mutant H197A Saccharomyces cerevisiae
0.00172
-
 a-factor derived synthetic farnesylated pentadecapeptide wild type Saccharomyces cerevisiae
0.00234
-
 a-factor derived synthetic farnesylated pentadecapeptide mutant C251A Saccharomyces cerevisiae
0.00326
-
 a-factor derived synthetic farnesylated pentadecapeptide mutant H196A Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-
membrane
-
 Saccharomyces cerevisiae 16020
-
membrane
-
 Arabidopsis thaliana 16020
-
membrane
-
 Caenorhabditis elegans 16020
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Caenorhabditis elegans
-
-
-
Drosophila melanogaster
-
-
-
Homo sapiens
-
-
-
Saccharomyces cerevisiae
-
 strain RC757, strain SM1068, strain SM3614, strain STY50
-
Schizosaccharomyces pombe
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a-factor derived synthetic farnesylated pentadecapeptide + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
CALM + H2O
-
 Homo sapiens ?
-
 ?
CALM + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
CALQ + H2O
-
 Homo sapiens ?
-
 ?
CALQ + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
CAMQ + H2O
-
 Homo sapiens ?
-
 ?
CAMQ + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
CASQ + H2O no cleavage when RCE1 is expressed from low copy plasmids or is overexpressed Arabidopsis thaliana ?
-
 ?
CASQ + H2O no cleavage when RCE1 is expressed from low copy plasmids or is overexpressed Caenorhabditis elegans ?
-
 ?
CASQ + H2O no cleavage when RCE1 is expressed from low copy plasmids, but overexpressed RCE1 appears to have marginal activity towards CASQ Saccharomyces cerevisiae ?
-
 ?
CSVM + H2O
-
 Homo sapiens ?
-
 ?
CSVM + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
CTLM + H2O
-
 Homo sapiens ?
-
 ?
CTLM + H2O one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed Saccharomyces cerevisiae ?
-
 ?
CTLM + H2O one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed Arabidopsis thaliana ?
-
 ?
CTLM + H2O one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed Caenorhabditis elegans ?
-
 ?
CTSM + H2O
-
 Homo sapiens ?
-
 ?
CTSM + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
CTVM + H2O
-
 Homo sapiens ?
-
 ?
CTVM + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
CVIA + H2O
-
 Homo sapiens ?
-
 ?
CVIA + H2O one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed Saccharomyces cerevisiae ?
-
 ?
CVIA + H2O one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed Arabidopsis thaliana ?
-
 ?
CVIA + H2O one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed Caenorhabditis elegans ?
-
 ?
additional information CTSQ motif poorly cleaved Homo sapiens ?
-
 ?
additional information CTSQ motif poorly cleaved Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
Ras-converting enzyme
-
 Drosophila melanogaster
Ras-converting enzyme
-
 Homo sapiens
Ras-converting enzyme
-
 Saccharomyces cerevisiae
Ras-converting enzyme
-
 Arabidopsis thaliana
Ras-converting enzyme
-
 Schizosaccharomyces pombe
Ras-converting enzyme
-
 Caenorhabditis elegans
RCE1
-
 Drosophila melanogaster
RCE1
-
 Homo sapiens
RCE1
-
 Saccharomyces cerevisiae
RCE1
-
 Arabidopsis thaliana
RCE1
-
 Schizosaccharomyces pombe
RCE1
-
 Caenorhabditis elegans
Rce1p
-
 Saccharomyces cerevisiae