Activating Compound | Comment | Organism | Structure |
---|---|---|---|
PA200 | activator protein | Spodoptera frugiperda | |
PA28 | activator protein | Spodoptera frugiperda |
Crystallization (Comment) | Organism |
---|---|
the 20S proteasome core particle forms stable complexes with one or two dimers of assembly chaperones PAC1-PAC2 and activators PA28gamma, PA200 in Spodoptera frugiperda Sf9 cells. Spontaneous detachment of 19S RP from isolated 26S proteasomes leaves core particles at different stages in the gate opening | Spodoptera frugiperda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spodoptera frugiperda | A0A2H1WB52 | - |
- |
Purification (Comment) | Organism |
---|---|
several forms of 20S proteasomes from extracts of Spodoptera frugiperda (Sf9) cells are separated using electrophoresis in a native polyacrylamide gel | Spodoptera frugiperda |
Subunits | Comment | Organism |
---|---|---|
More | presence of 7 alpha-type and 7 beta-type subunits in the 20S complex | Spodoptera frugiperda |
Synonyms | Comment | Organism |
---|---|---|
SFRICE_003436 | - |
Spodoptera frugiperda |
General Information | Comment | Organism |
---|---|---|
physiological function | the 19S regulatory particle in 26S proteasomes performs stepwise substrate unfolding and opens the chamber gate in an ATP-dependent manner. Spontaneous dissociation of the regulatory particle in isolated 26S proteasomes leaves core particles with different gate sizes related presumably to different stages in the gate opening | Spodoptera frugiperda |