Literature summary for 3.4.25.1 extracted from

Kaake, R.M.; Milenkovic, T.; Przulj, N.; Kaiser, P.; Huang, L.
Characterization of cell cycle specific protein interaction networks of the yeast 26S proteasome complex by the QTAX strategy (2010), J. Proteome Res., 9, 2016-2029.

Protein Variants

Protein Variants	Comment	Organism
additional information	development of an integrated proteomic approach, QTAX, for quantitative analysis of tandem affinity purified in vivo cross-linked protein complexes to capture protein interactions of all natures in a single analysis, overview. Investigation of cell cycle specific proteasome interaction networks. Wild-type and RPN11-HBH cells are synchronized in three phases (G1, S, and M) before cross-linking and tandem affinity purification	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
development of an integrated proteomic approach, QTAX, for quantitative analysis of tandem affinity purified in vivo cross-linked protein complexes to capture protein interactions of all natures in a single analysis, overview. Investigation of cell cycle specific proteasome interaction networks. Wild-type and RPN11-HBH cells are synchronized in three phases (G1, S, and M) before cross-linking and tandem affinity purification using nickel affinity resin and streptavidin beads after lysis using buffer containing 8 M urea, 300 mM NaCl, 50 mM NaH2PO4, 0.5% Igepal, 20 mM imidazole, and 1 mM PMSF, pH 8.5	Saccharomyces cerevisiae

Purification (Comment)

Organism

development of an integrated proteomic approach, QTAX, for quantitative analysis of tandem affinity purified in vivo cross-linked protein complexes to capture protein interactions of all natures in a single analysis, overview. Investigation of cell cycle specific proteasome interaction networks. Wild-type and RPN11-HBH cells are synchronized in three phases (G1, S, and M) before cross-linking and tandem affinity purification using nickel affinity resin and streptavidin beads after lysis using buffer containing 8 M urea, 300 mM NaCl, 50 mM NaH2PO4, 0.5% Igepal, 20 mM imidazole, and 1 mM PMSF, pH 8.5

Saccharomyces cerevisiae

General Information

General Information	Comment	Organism
additional information	development of an integrated proteomic approach, QTAX, for quantitative analysis of tandem affinity purified in vivo cross-linked protein complexes to capture protein interactions of all natures in a single analysis, overview. Investigation of cell cycle specific proteasome interaction networks	Saccharomyces cerevisiae