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Literature summary for 3.4.25.1 extracted from
- Purification and initial characterization of the proteasome from the higher plant Spinacia oleracea (1992), J. Biol. Chem., 267, 21678-21684.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| poly-L-lysine | stimulates chymotrypsin-, trypsin- and Staphylococcus aureus V8-like activities | Spinacia oleracea |  |
| SDS | stimulates chymotrypsin-like activity and Staphylococcus aureus V8 like activity | Spinacia oleracea | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chymostatin | inhibits chymotrypsin-like activity | Spinacia oleracea | |
| leupeptin | inhibits trypsin-like activity | Spinacia oleracea | |
| N-ethylmaleimide | - |
Spinacia oleracea | |
| phenylmethylsulfonyl fluoride | inhibits trypsin-like activity | Spinacia oleracea | |
| SDS | inhibits trypsin-like activity | Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 850000 | - |
gel filtration | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
spinach | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| cleavage of peptide bonds with very broad specificity | Staphylococcus aureus V8 protease like activity | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.022 | - |
- |
Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | - |
Spinacia oleracea | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | multiple subunits from 21000-32000, SDS-PAGE | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
chymotrypsin-like activity | Spinacia oleracea |
| 9 | - |
Staphylococcus aureus V8 like activity | Spinacia oleracea |
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