Any feedback?
Literature summary for 3.4.24.B9 extracted from
- Metalloprotease-disintegrin MDC9: intracellular maturation and catalytic activity (1999), J. Biol. Chem., 274, 3531-3540.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Protein kinase C | phosphorylation of the enzyme by protein kinase C activates the enzyme, phosphorylation is stimualted by phorbol 12-myristate 13-acetate | Mus musculus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of soluble enzyme form, nucleotides 1-1253, with a Myc-epitope in COS-7 cells | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | site-directed mutagenesis of the furin cleavage site and of the cysteine-switch region results in highly reduced precursor processing, construction of a deletion mutant resulting in a soluble enzyme form | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Mus musculus |  |
| batimastat | - |
Mus musculus | |
| brefeldin A | blocks processing of the enzyme precursor by blocking the vesicle budding in the endoplasmic reticulum | Mus musculus | |
| CGS 27023 | - |
Mus musculus | |
| coban | complete blockage of enzyme processing at 0.025 mg/ml | Mus musculus | |
| marimastat | - |
Mus musculus | |
| PKVCGYLK | inhibition less tan 20% at 0.1 mM | Mus musculus | |
| PLKCGVSN | inhibition less tan 20% at 0.1 mM | Mus musculus | |
| PLRCGVSN | inhibition less tan 20% at 0.1 mM | Mus musculus | |
| QGGCADHS | inhibition less tan 20% at 0.1 mM | Mus musculus | |
| QGLCGSQH | inhibition less tan 20% at 0.1 mM | Mus musculus | |
| TNF-alpha protease inhibitor | - |
Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | anchored on the cell surface | Mus musculus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | metalloprotease | Mus musculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
x * 84000, processed enzyme, x * 12000, enzyme proform, SDS-PAGE | Mus musculus |
| 84000 | - |
x * 84000, processed enzyme, x * 12000, enzyme proform, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mus musculus | regulation of metalloprotease desintegrins via a cysteine-switch mechanism | ? | - |
? | |
| proteins + H2O | Mus musculus | - |
peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | N-glycosylation of endopeptidase H-sensitive precursor and mature enzyme | Mus musculus |
| proteolytic modification | cleavage of pro-domain by furin-type pro-protein convertase before the protein is secreted to the cell surface | Mus musculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble enzyme from COS-7 cells | Mus musculus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| EVHHQKLVFFAE + H2O | peptides derived from beta-amyloid precursor protein, comprises the predicted cleavage site | Mus musculus | EVHH + QKLVFFAE | - |
? | |
| insulin B chain + H2O | cleavage sites are Tyr16-Leu17 and Tyr26-Thr27 | Mus musculus | 3 peptides | - |
? | |
| additional information | regulation of metalloprotease desintegrins via a cysteine-switch mechanism | Mus musculus | ? | - |
? | |
| proteins + H2O | - |
Mus musculus | peptides | - |
? | |
| SMAPGAVHLPQP + H2O | peptides derived from P75 TNF-receptor, comprises the predicted cleavage site | Mus musculus | SMAPGAVH + LPQP | - |
? | |
| SPLAQAVRSSSR + H2O | peptides derived from TNF-alpha, comprises the predicted cleavage site | Mus musculus | SPLA + QAVRSSSR | - |
? | |
| SPLAQAVRSSSR + H2O | peptides derived from TNF-alpha, comprises the predicted cleavage site | Mus musculus | SPLAQAVRS + SSR | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 84000, processed enzyme, x * 12000, enzyme proform, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| M12.209 | Merops-ID | Mus musculus |
| MDC9 | - |
Mus musculus |
| metallo protease desintegrin 9 | - |
Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Mus musculus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000014 | - |
batimastat | pH 7.5 | Mus musculus | |
| 0.000017 | - |
TNF-alpha protease inhibitor | pH 7.5 | Mus musculus | |
| 0.000054 | - |
CGS 27023 | pH 7.5 | Mus musculus | |
| 0.00027 | - |
marimastat | pH 7.5 | Mus musculus | |
| 0.004 | - |
PKVCGYLK | pH 7.5 | Mus musculus | |
| 0.01 | - |
PLRCGVSN | pH 7.5 | Mus musculus | |
| 0.037 | - |
PLKCGVSN | pH 7.5 | Mus musculus | |
| 0.15 | - |
QGLCGSQH | pH 7.5 | Mus musculus | |
| 0.15 | - |
QGGCADHS | pH 7.5 | Mus musculus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
