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Literature summary for 3.4.24.B6 extracted from
- On-column activation of bovine recombinant metalloproteinase 20 (2006), Anal. Biochem., 356, 291-293.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Bos taurus |
General Stability
| General Stability | Organism |
|---|---|
| the addition of 1 mM EDTA into activation buffer can protect the 55 kDa proenzyme from autolysis and degradation | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | neither calcium nor magnesium alone can effectively activate the proenzyme | Bos taurus | |
| Zn2+ | 1 mM, activates effectively | Bos taurus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
pro-enzyme, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ameloblast | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amelogenin + H2O | - |
Bos taurus | ? | - |
? | |
| Mca-PLGL-Dpa-AR + H2O | - |
Bos taurus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| enamelysin | - |
Bos taurus |
| matrix metalloproteinase 20 | - |
Bos taurus |
| MMP-20 | - |
Bos taurus |
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Release 2023.1 (January 2023)
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