Cloned (Comment) | Organism |
---|---|
overexpression of His-atgged pro-enzyme in Escherichia coli BL21(DE3) | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | in enamel matrix of teeth | Bos taurus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylogenin + H2O | Bos taurus | involved in tooth formation | ? | - |
? | |
proteins + H2O | Bos taurus | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | O18767 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequential autolysis, activation of recombinant proform by urea | Bos taurus |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged pro-enzyme from Escherichia coli | Bos taurus |
Renatured (Comment) | Organism |
---|---|
renaturation from inclusion bodies after overexpression in Escherichia coli | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ameloblast | secretion | Bos taurus | - |
tooth | - |
Bos taurus | - |
tooth enamel | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylogenin + H2O | - |
Bos taurus | ? | - |
? | |
amylogenin + H2O | involved in tooth formation | Bos taurus | ? | - |
? | |
casein + H2O | - |
Bos taurus | ? | - |
? | |
leucine-rich amylogenin peptide + H2O | corresponds to residues 181-188 of amylogenin, recombinant enzyme, cleavage site is P186-A187 | Bos taurus | leucine-rich amylogenin residues 181-186 + leucine-rich amylogenin residues 187-188 | - |
? | |
proteins + H2O | - |
Bos taurus | peptides | - |
? | |
tyrosine-rich amylogenin peptide + H2O | corresponds to residues 36-49 of amylogenin, recombinant enzyme, cleavage site is W45-L46 | Bos taurus | tyrosine-rich amylogenin residues 36-45 + tyrosine-rich amylogenin residues 46-49 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enamel metalloproteinase | - |
Bos taurus |
enamelysin | - |
Bos taurus |
M10.019 | Merops-ID | Bos taurus |
MMP-20 | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at, zymography with casein at room temperature | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bos taurus |