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Literature summary for 3.4.24.B4 extracted from

  • Knaeuper, V.; Lopez-Otin, C.; Smith, B.; Knight, G.; Murphy, G.
    Biochemical characterization of human collagenase-3 (1996), J. Biol. Chem., 271, 1544-1550.
    View publication on PubMed
Search for more literature for 3.4.24.B4

Cloned(Commentary)

Cloned (Comment) Organism
expression of procollagenase 3 in NSO mouse myeloma cells, secretion to the medium Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
hydroxamic acid-based inhibitor CT1399
-
 Homo sapiens
hydroxamic acid-based inhibitor CT1847
-
 Homo sapiens
tissue matrix metalloproteinase inhibitor TIMP-1 1:1 stoichiometric fashion Homo sapiens
tissue matrix metalloproteinase inhibitor TIMP-2 1:1 stoichiometric fashion Homo sapiens
tissue matrix metalloproteinase inhibitor TIMP-3 1:1 stoichiometric fashion Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 48000, mature enzyme, SDS-PAGE Homo sapiens
50000
-
 x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 48000, mature enzyme, SDS-PAGE Homo sapiens
60000
-
 x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 48000, mature enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Homo sapiens enzyme is involved in tumor progression, significant role in the turnover of connective tissue matrix constituents peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylated Homo sapiens
proteolytic modification cleavage by trypsin is not effective, processing of the 60 kDa procollagenase 3 by stromelysin or by p-aminophenylmercuric acetate to form an intermediate of 50 kDa, further processing by cleavage of the peptide bond Glu84-Tyr85 resuls in the active 48 kDa mature form, overview Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant procollagenase 3 from NSO mouse myeloma cell culture medium Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
breast carcinoma cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin)-4-yl-acetyl-Pro-Cha-Gly-norvaline-His-Ala-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-NH2 + H2O
-
 Homo sapiens (7-methoxycoumarin)-4-yl-acetyl-Pro-Cha-Gly + norvaline-His-Ala-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-NH2
-
 ?
(7-methoxycoumarin)-4-yl-acetyl-Pro-Leu-Gly-Leu-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2 + H2O
-
 Homo sapiens (7-methoxycoumarin)-4-yl-acetyl-Pro-Leu-Gly + Leu-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2
-
 ?
alpha1-Antichymotrypsin + H2O cleavage of peptide bond Ala362-Leu363 Homo sapiens ?
-
 ?
Collagen type I + H2O fibrillar, 5 to 6fold less active compared to substrate collagen type II Homo sapiens ?
-
 ?
collagen type II + H2O soluble, preferred substrate Homo sapiens ?
-
 ?
Collagen type III + H2O 5 to 6fold less active compared to substrate collagen type II Homo sapiens ?
-
 ?
Gelatin + H2O
-
 Homo sapiens ?
-
 ?
additional information no activity with antithrombin III Homo sapiens ?
-
 ?
plasminogen activator inhibitor 2 + H2O
-
 Homo sapiens ?
-
 ?
protein + H2O
-
 Homo sapiens peptides
-
 ?
protein + H2O enzyme is involved in tumor progression, significant role in the turnover of connective tissue matrix constituents Homo sapiens peptides
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 48000, mature enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
M10.013 Merops-ID Homo sapiens
matrix metalloproteinase-13
-
 Homo sapiens
MMP-13
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information
-
 Homo sapiens