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Literature summary for 3.4.24.B39 extracted from

  • Jiang, W.; Ma, T.; Su, X.; Qiu, P.; Yan, G.
    Enzymatic activities and functional characterization of a novel recombinant snake venom proteinase from Agkistrodon acutus (2009), Biochimie, 91, 277-287.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 83.8% residual activity at 3.2 mM Deinagkistrodon acutus
dithiothreitol incubation with 5 mM dithiothreitol results in 34% inhibition of azocasein hydrolysis Deinagkistrodon acutus
EDTA 29.6% residual activitty at 5 mM Deinagkistrodon acutus
additional information aprotinin, and trypsin inhibitor SBTI do not affect the enzymatic activity Deinagkistrodon acutus
phenylmethylsulfonyl fluoride 1.2% residual activitty at 2 mM Deinagkistrodon acutus
Zn2+ 85.1% residual activity at 3.2 mM Deinagkistrodon acutus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5382
-
N-(4-tosyl)-Gly-Pro-Lys-4-nitroanilide at pH 8.0 and 37°C Deinagkistrodon acutus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Ca2+, Mg2+, Na+, and K+ do not affect proteolytic activity Deinagkistrodon acutus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fibrin + H2O Deinagkistrodon acutus
-
?
-
?
Fibrinogen + H2O Deinagkistrodon acutus the enzyme preferentially cleaves the Aalpha-chain of fibrinogen, followed by the Bbeta-chain and finally the gamma chain ?
-
?
Fibronectin + H2O Deinagkistrodon acutus
-
?
-
?
Oxidized insulin B-chain + H2O Deinagkistrodon acutus the enzyme preferentially cleaves oxidized insulin B-chain at the site of Val12-Glu13, Leu15-Tyr16, and Phe24-Phe25 ?
-
?
plasmin + H2O Deinagkistrodon acutus
-
?
-
?
type IV collagen Deinagkistrodon acutus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Deinagkistrodon acutus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Deinagkistrodon acutus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
Deinagkistrodon acutus ?
-
?
casein + H2O
-
Deinagkistrodon acutus ?
-
?
D-Pro-Phe-Arg-4-nitroanilide + H2O synthetic substrate P7959 Deinagkistrodon acutus D-Pro-Phe-Arg + 4-nitroaniline
-
?
Fibrin + H2O
-
Deinagkistrodon acutus ?
-
?
Fibrinogen + H2O the enzyme preferentially cleaves the Aalpha-chain of fibrinogen, followed by the Bbeta-chain and finally the gamma chain Deinagkistrodon acutus ?
-
?
Fibronectin + H2O
-
Deinagkistrodon acutus ?
-
?
N-(4-tosyl)-Gly-Pro-Lys-4-nitroanilide + H2O best synthetic substrate, T6140 Deinagkistrodon acutus N-(4-tosyl)-Gly-Pro-Lys + 4-nitroaniline
-
?
N-benzoyl-Pro-Phe-Arg-4-nitroanilide + H2O synthetic substrate B2133 Deinagkistrodon acutus N-benzoyl-Pro-Phe-Arg + 4-nitroaniline
-
?
Oxidized insulin B-chain + H2O the enzyme preferentially cleaves oxidized insulin B-chain at the site of Val12-Glu13, Leu15-Tyr16, and Phe24-Phe25 Deinagkistrodon acutus ?
-
?
plasmin + H2O
-
Deinagkistrodon acutus ?
-
?
type IV collagen
-
Deinagkistrodon acutus ?
-
?

Synonyms

Synonyms Comment Organism
fibrinogenase
-
Deinagkistrodon acutus
rFII
-
Deinagkistrodon acutus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 the enzyme shows highest activity between 30 and 50°C. Activity drops sharply under higher temperatures, with a complete loss of activity occurring above 60°C Deinagkistrodon acutus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.419
-
N-(4-tosyl)-Gly-Pro-Lys-4-nitroanilide at pH 8.0 and 37°C Deinagkistrodon acutus

pH Range

pH Minimum pH Maximum Comment Organism
7 10.5 the enzyme shows highest activity between pH 7.0 and 10.5. Activity drops sharply under more alkaline or acidic conditions with a complete loss of activity occurring at pH values 4.0 and 11.5 Deinagkistrodon acutus

pI Value

Organism Comment pI Value Maximum pI Value
Deinagkistrodon acutus isoelectric focusing
-
9

General Information

General Information Comment Organism
physiological function the enzyme dose-dependently inhibits platelet aggregation triggered by ADP (0.005 mM) in human platelet-rich plasma. The enzyme cannot activate plasminogen Deinagkistrodon acutus