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Literature summary for 3.4.24.B34 extracted from

  • Leonardi, A.; Sajevic, T.; Latinovic, Z.; Pungercar, J.; Lang Balija, M.; Trampus; Bakija, A.; Vidmar, R.; Halassy, B.; Krizaj, I.
    Structural and biochemical characterisation of VaF1, a P-IIIa fibrinogenolytic metalloproteinase from Vipera ammodytes ammodytes venom (2015), Biochimie, 109, 78-87.
    View publication on PubMed
Search for more literature for 3.4.24.B34

Application

Application Comment Organism
medicine in standard experimental conditions, enzyme VaF1 is not recognised by antiserum against the whole venom, so it can contribute to postserotherapy complications, such as ineffective blood coagulation, in the envenomed patient Vipera ammodytes ammodytes

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Vipera ammodytes ammodytes

Inhibitors

Inhibitors Comment Organism Structure
EDTA pre-incubation of the enzyme with EDTA blocks the fibrinogenolytic activity Vipera ammodytes ammodytes
additional information pefabloc, an inhibitor of serine proteases, has no effect on the enzyme proteolytic activity Vipera ammodytes ammodytes

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Vipera ammodytes ammodytes
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49735
-
 1 * 49735, mass spectrometry, SDS-PAGE and native PAGE Vipera ammodytes ammodytes
49740
-
 mass spectrometry, SDS-PAGE and native PAGE Vipera ammodytes ammodytes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
factor X + H2O Vipera ammodytes ammodytes partial hydrolysis ?
-
 ?
Human fibrinogen + H2O Vipera ammodytes ammodytes
-
 ?
-
 ?
additional information Vipera ammodytes ammodytes hydrolysis of fibrinogen, factor X, prothrombin and plasminogen, plasma proteins involved in blood coagulation ?
-
 ?
plasminogen + H2O Vipera ammodytes ammodytes partial hydrolysis ?
-
 ?
prothrombin + H2O Vipera ammodytes ammodytes partial hydrolysis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Vipera ammodytes ammodytes A0A0B4U9L8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme contains two consensus N-glycosylation sites, the extent of its glycosylation is 5.2% of the total molecular mass. Deglycosylation by peptide N-glycosidase F. Deglycosylation reduces the molecular weight by 3.0 kDa Vipera ammodytes ammodytes

Purification (Commentary)

Purification (Comment) Organism
native enzyme by several steps including cation exchange chromatography, dialysis and anion exchange chromatography Vipera ammodytes ammodytes

Reaction

Reaction Comment Organism Reaction ID
Cleavage of the Lys413-/-Leu414 bond of alpha-chain of human fibrinogen. Cleavage of Ala14-/-Leu15 and Tyr16-/-Leu17 in insulin B chain. Non-hemorrhagic proteinase no cleavage of fibrin, fibrinogen beta-chain or fibrinogen gamma-chain Vipera ammodytes ammodytes
a

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Vipera ammodytes ammodytes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bovine factor X + H2O slow degradation. The heavy chain offactor X is more susceptible to hydrolysis by the enzyme than the light chain whose hydrolysis is observed only after prolonged incubation Vipera ammodytes ammodytes ?
-
 ?
bovine fibronectin + H2O
-
 Vipera ammodytes ammodytes ?
-
 ?
bovine plasminogen + H2O slow degradation. Very limited proteolysis, giving rise to multiple products with molecular masses between 40 and 60 kDa Vipera ammodytes ammodytes ?
-
 ?
bovine prothrombin + H2O slow degradation. Two major cleavage products, of 60 and 28 kDa, accumulate after extended incubation. N-terminal sequencing shows them to be prethrombin-1 and fragment 1, indicating the cleavage of prothrombin at the Ser157-/-Gly158 bond Vipera ammodytes ammodytes ?
-
 ?
factor X + H2O partial hydrolysis Vipera ammodytes ammodytes ?
-
 ?
Human fibrinogen + H2O
-
 Vipera ammodytes ammodytes ?
-
 ?
human fibrinogen alpha-chain + H2O cleavage of the Lys413-/-Leu414 bond of alpha-chain of fibrinogen. Fibrinogen beta- and gamma-chains remain intact even after 3 h incubation Vipera ammodytes ammodytes ?
-
 ?
human type collagen IV + H2O
-
 Vipera ammodytes ammodytes ?
-
 ?
insulin B chain + H2O cleavage of two peptide bonds is detected, Ala14-/-Leu15 and Tyr16-/-Leu17 Vipera ammodytes ammodytes ?
-
 ?
additional information hydrolysis of fibrinogen, factor X, prothrombin and plasminogen, plasma proteins involved in blood coagulation Vipera ammodytes ammodytes ?
-
 ?
additional information non-hemorrhagic proteinase. No degradation of fibrin. Laminin resists hydrolysis by the enzyme, even after 24 h incubation Vipera ammodytes ammodytes ?
-
 ?
Nidogen + H2O hydrolysis at the Ser322-/-Phe323 bond Vipera ammodytes ammodytes ?
-
 ?
plasminogen + H2O partial hydrolysis Vipera ammodytes ammodytes ?
-
 ?
prothrombin + H2O partial hydrolysis Vipera ammodytes ammodytes ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 49735, mass spectrometry, SDS-PAGE and native PAGE Vipera ammodytes ammodytes

Synonyms

Synonyms Comment Organism
VaF1
-
 Vipera ammodytes ammodytes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Vipera ammodytes ammodytes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Vipera ammodytes ammodytes

pI Value

Organism Comment pI Value Maximum pI Value
Vipera ammodytes ammodytes isoelectric focussing
-
 5.8