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Literature summary for 3.4.24.B17 extracted from
- Coupled kinetics of ATP and peptide hydrolysis by Escherichia coli FtsH protease (2003), Biochemistry, 42, 10843-10852.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ftsH gene, expression as His-tagged enzyme in strain BL21(DE3) | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Escherichia coli |  |
| additional information | no inhibition by phenylmethylsulfonylfluoride | Escherichia coli | |
| o-phenanthroline | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Escherichia coli | |
| 0.021 | - |
ATP | with FGH-NO2FFAF-methoxy, pH 8.0, 37°C | Escherichia coli | |
| 0.023 | - |
protein sigma32 | pH 8.0, 42°C | Escherichia coli | |
| 0.023 | - |
ATP | with protein sigma32, pH 8.0, 37°C | Escherichia coli | |
| 0.104 | - |
CTP | pH 8.0, 37°C | Escherichia coli | |
| 7.3 | - |
GTP | pH 8.0, 37°C | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | integral, schematic model of structure and arrangement in the membane | Escherichia coli | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | dependent on | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the large complexes exhibit either ATPase and protease activity, while smaller ones do not | Escherichia coli |
| 70000 | - |
x * 70000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein + H2O | Escherichia coli | involved in the degradation of regulatory proteins and uncomplexed subunits of membrane protein complexes | peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme, solubilization by N-lauylsarcosine, refolding in presence of ATP, Mg2+ and Zn2+, and purification to near homogeneity | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| proteolytic degradation of proteins | mechanism | Escherichia coli |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant enzyme, solubilization by N-lauylsarcosine, refolding in presence of ATP, Mg2+ and Zn2+, and purification to near homogeneity, the refolded enzyme has properties similar to the overexpressed solubilized one | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0036 | - |
purified recombinant refolded enzyme, substrate protein sigma32 | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + H2O | - |
Escherichia coli | ? | - |
? | |
| FGH-(NO2)FFAF-methyl ester + H2O | exclusively cleaved at the (NO2)Phe-Phe bond | Escherichia coli | FGH-(NO2)F + Phe-Ala-Phe-methyl ester | - |
? | |
| GTP + H2O | - |
Escherichia coli | ? | - |
? | |
| additional information | large complexes exhibit either ATPase and protease activity, while smaller ones do not | Escherichia coli | ? | - |
? | |
| protein + H2O | - |
Escherichia coli | peptides | - |
? | |
| protein + H2O | involved in the degradation of regulatory proteins and uncomplexed subunits of membrane protein complexes | Escherichia coli | peptides | - |
? | |
| protein SecY + H2O | uncomplexed subunit of the protein translocase | Escherichia coli | ? | - |
? | |
| protein sigma32 + H2O | regulatory protein | Escherichia coli | peptides | approximately 10 peptides with MW below 3 kDa | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | x * 70000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FtsH | - |
Escherichia coli |
| FtsH protease | - |
Escherichia coli |
| M41.001 | Merops-ID | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
protein sigma32 | pH 8.0, 42°C | Escherichia coli | |
| 0.023 | - |
ATP | with FGH-NO2FFAF-methoxy, pH 8.0, 37°C | Escherichia coli | |
| 0.3 | - |
ATP | with protein sigma32, pH 8.0, 37°C | Escherichia coli | |
| 0.32 | - |
CTP | pH 8.0, 37°C | Escherichia coli | |
| 0.53 | - |
GTP | pH 8.0, 37°C | Escherichia coli | |
| 6.08 | - |
GTP | pH 8.0, 37°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2',3'-dideoxy-ATP | - |
Escherichia coli | |
| 2',3'-dideoxy-CTP | - |
Escherichia coli | |
| 2'-deoxy-ATP | - |
Escherichia coli | |
| 3'-deoxy-ATP | - |
Escherichia coli | |
| 7-daza-2'-deoxy-ATP | low activity | Escherichia coli | |
| ATP | - |
Escherichia coli | |
| ATPalphaS | low activity | Escherichia coli | |
| CTP | - |
Escherichia coli | |
| dCTP | - |
Escherichia coli | |
| additional information | no activity with ATPgammaS, absolutely dependent on nucleoside 5'-triphosphates like ATP or CTP, not GTP or UTP, or derivatives | Escherichia coli |
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