Literature summary for 3.4.24.B16 extracted from

Peters, J.E.; Park, S.J.; Darzins, A.; Freck, L.C.; Saulnier, J.M.; Wallach, J.M.; Galloway, D.R.
Further studies on Pseudomonas aeruginosa LasA: analysis of specificity (1992), Mol. Microbiol., 6, 1155-1162.

Search for more literature for 3.4.24.B16

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	no inhibition by EDTA and o-phenanthroline	Pseudomonas aeruginosa
N-tosyl-L-lysine chloromethyl ketone	-	Pseudomonas aeruginosa
Phenylmethylsulfonylfluoride	-	Pseudomonas aeruginosa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	active fragment	Pseudomonas aeruginosa	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	enzyme is no metalloprotease	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
protein + H2O	Pseudomonas aeruginosa	-	peptides	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
proteolytic degradation of proteins	enzyme might be a modified serine protease with a His residue in the active site, specifically involved in degradation of elastin	Pseudomonas aeruginosa	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-casein + H2O	only 1 cleavage site: in the sequence NKKIEKFQphosphorylated-S between Lys and Ile, serine protease activity	Pseudomonas aeruginosa	beta-casein + 30 amino acid residues fragment	-	?
Elastin + H2O	cleavage of elastin by the enzyme possibly results in the unfolding or disruption of the complex elastin structure, thus providing more access to elastase and other proteases, elastolytic activity in absence of elastase, enzyme also enhances the elastolytic activity of elastase together with alkaline phophatase	Pseudomonas aeruginosa	?	-	?
additional information	no activity with tosyl-Gly-Pro-Lys-4-nitroanilide, enzyme activates elastase, and the elastolytic, not the proteolytic, activity of thermolysin, human neutrophil elastase, proteinase K, by interacting with the elastin substrate rather than the other enzymes	Pseudomonas aeruginosa	?	-	?
protein + H2O	-	Pseudomonas aeruginosa	peptides	-	?

Synonyms

Synonyms	Comment	Organism
LasA	-	Pseudomonas aeruginosa
M23.002	Merops-ID	Pseudomonas aeruginosa
staphylolysin	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)