Literature summary for 3.4.24.B14 extracted from

Takamatsu, K.; Ikeda, T.; Haruta, M.; Matsumura, K.; Ogi, Y.; Nakagata, N.; Uchino, M.; Ando, Y.; Nishimura, Y.; Senju, S.
Degradation of amyloid beta by human induced pluripotent stem cell-derived macrophages expressing neprilysin-2 (2014), Stem Cell Res., 13, 442-453.

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Cloned(Commentary)

Cloned (Comment)	Organism
neprilysin-2 expression in human induced pluripotent stem (iPS) cell-derived macrophage-like cells which leads to secretion of the enzyme, to reduced level of soluble amyloid beta oligomer in the culture medium, and to alleviated neurotoxicity of amyloid beta. Intracerebrally administration of the iPS-ML/NEP2 cells to 5XFAD mice leading to significant reduction in the level of amyloid beta in the brain interstitial fluid. 5XFAD mice harbor three mutations in the amyloid precursor protein (APP) gene and two in the presenilin-1 (PS1) gene and are a mouse model of Alzheimer's disease	Homo sapiens

Cloned (Comment)

Organism

neprilysin-2 expression in human induced pluripotent stem (iPS) cell-derived macrophage-like cells which leads to secretion of the enzyme, to reduced level of soluble amyloid beta oligomer in the culture medium, and to alleviated neurotoxicity of amyloid beta. Intracerebrally administration of the iPS-ML/NEP2 cells to 5XFAD mice leading to significant reduction in the level of amyloid beta in the brain interstitial fluid. 5XFAD mice harbor three mutations in the amyloid precursor protein (APP) gene and two in the presenilin-1 (PS1) gene and are a mouse model of Alzheimer's disease

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
phosphoramidon	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	soluble-secreted protease isoform	Homo sapiens	-	-
membrane	membrane-bound protease isoform	Homo sapiens	16020	-
additional information	neprilysin-2 has two alternatively spliced forms, a membrane-bound and soluble-secreted variant	Homo sapiens	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	a metallo-endopeptidase-like protein	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
amyloid beta peptide + H2O	Homo sapiens	-	?	-	?
amyloid beta peptide1-42 + H2O	Homo sapiens	-	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q495T6	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
amyloid beta peptide + H2O	-	Homo sapiens	?	-	?
amyloid beta peptide1-42 + H2O	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
membrane metallo-endopeptidase-like protein	-	Homo sapiens
MMEL	-	Homo sapiens
NEP2	-	Homo sapiens
neprilysin-2	-	Homo sapiens
SEP	-	Homo sapiens
soluble-secreted endopeptidase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens