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Literature summary for 3.4.24.B14 extracted from

  • Whyteside, A.R.; Turner, A.J.
    Human neprilysin-2 (NEP2) and NEP display distinct subcellular localisations and substrate preferences (2008), FEBS Lett., 582, 2382-2386.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of both isoforms, NEP2 and NEP2DELTA Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
phosphoramidon approximately 8-fold more potent against neprilysin (EC 3.4.24.11) than neprilysin-2 Homo sapiens
thiorphan far more potent against neprilysin (EC 3.4.24.11) than neprilysin-2 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane NEP2 exists as a membrane-bound and soluble enzyme, isoform NEP2DELTA exists as two membrane-bound glycoforms glycoforms, localised to the ER and plasma membrane Homo sapiens 16020
-
soluble NEP2 exists as a membrane-bound and soluble enzyme Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q495T6 soluble form, NEP2(s)
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein isoform NEP2DELTA exists as two membrane-bound glycoforms glycoforms, localised to the ER and plasma membrane Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O hydrolyzed to 92% Homo sapiens ?
-
?
bradykinin + H2O hydrolyzed to 57% Homo sapiens ?
-
?
additional information neprilysin-2 shows a much restriceted substrate specificity compared to neprilysin Homo sapiens ?
-
?
NEP fluorogenic substrate + H2O hydrolyzed to 39% Homo sapiens ?
-
?
Substance P + H2O completely hydrolyzed Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
NEP2 NEP2 exists as two alternatively spliced isoforms, NEP2 and NEP2DELTA Homo sapiens
NEP2(s) soluble Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000169
-
-
Homo sapiens phosphoramidon
0.0221
-
-
Homo sapiens thiorphan