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Literature summary for 3.4.24.B14 extracted from

  • Bland, N.D.; Thomas, J.E.; Audsley, N.; Shirras, A.D.; Turner, A.J.; Isaac, R.E.
    Expression of NEP2, a soluble neprilysin-like endopeptidase, during embryogenesis in Drosophila melanogaster (2007), Peptides, 28, 127-135.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in S2 cells Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
phosphoramidon
-
Drosophila melanogaster
thiorphan
-
Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Drosophila melanogaster 5737
-
membrane
-
Drosophila melanogaster 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
deglycosylated enzyme, SDS-PAGE Drosophila melanogaster
100000
-
soluble enzyme, SDS-PAGE Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-
Drosophila melanogaster OregonR
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Drosophila melanogaster

Purification (Commentary)

Purification (Comment) Organism
Phenyl-Sepharose column chromatography and HiTrap Q HP column chromatography Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Drosophila melanogaster
-
malpighian tubule
-
Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Drosophila tachykinin-1 + H2O 3% activity compared to Locusta tachykinin-1 Drosophila melanogaster APTSS + FIGMR-amide
-
?
Drosophila tachykinin-1 + H2O 3% activity compared to Locusta tachykinin-1 Drosophila melanogaster OregonR APTSS + FIGMR-amide
-
?
Drosophila tachykinin-2 + H2O 33% activity compared to Locusta tachykinin-1 Drosophila melanogaster APLAFYG + Leu-Arg-amide
-
?
Drosophila tachykinin-2 + H2O 33% activity compared to Locusta tachykinin-1 Drosophila melanogaster OregonR APLAFYG + Leu-Arg-amide
-
?
Drosophila tachykinin-3 + H2O 1% activity compared to Locusta tachykinin-1 Drosophila melanogaster APTG + FTGMR-amide
-
?
Drosophila tachykinin-3 + H2O 1% activity compared to Locusta tachykinin-1 Drosophila melanogaster OregonR APTG + FTGMR-amide
-
?
Drosophila tachykinin-4 + H2O 8% activity compared to Locusta tachykinin-1 Drosophila melanogaster APVNS + FVGMR-amide
-
?
Drosophila tachykinin-4 + H2O 8% activity compared to Locusta tachykinin-1 Drosophila melanogaster OregonR APVNS + FVGMR-amide
-
?
Drosophila tachykinin-5 + H2O 2% activity compared to Locusta tachykinin-1 Drosophila melanogaster APNG + FLGMR-amide
-
?
Drosophila tachykinin-5 + H2O 2% activity compared to Locusta tachykinin-1 Drosophila melanogaster OregonR APNG + FLGMR-amide
-
?
Drosophila tachykinin-6 + H2O 17% activity compared to Locusta tachykinin-1 Drosophila melanogaster QQR + Phe + ADFNSKFVAVR-amide
-
?
Locusta tachykinin-1 + H2O 100% activity Drosophila melanogaster GPSGFYG + Val-amide
-
?

Synonyms

Synonyms Comment Organism
NEP2
-
Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Drosophila melanogaster

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8 activity is drastically reduced below pH 6.5 and above pH 8.0 Drosophila melanogaster

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0005
-
membrane-bound enzyme, in 0.1 M HEPES, 0.01 mM captopril, at pH 7 and 35°C Drosophila melanogaster thiorphan
0.002
-
soluble enzyme, in 0.1 M HEPES, 0.01 mM captopril, at pH 7 and 35°C Drosophila melanogaster phosphoramidon
0.003
-
membrane-bound enzyme, in 0.1 M HEPES, 0.01 mM captopril, at pH 7 and 35°C Drosophila melanogaster phosphoramidon
0.08
-
soluble enzyme, in 0.1 M HEPES, 0.01 mM captopril, at pH 7 and 35°C Drosophila melanogaster thiorphan