Cloned (Comment) | Organism |
---|---|
expressed in S2 cells | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphoramidon | - |
Drosophila melanogaster | |
thiorphan | - |
Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Drosophila melanogaster | 5737 | - |
membrane | - |
Drosophila melanogaster | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
deglycosylated enzyme, SDS-PAGE | Drosophila melanogaster |
100000 | - |
soluble enzyme, SDS-PAGE | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Drosophila melanogaster OregonR | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Drosophila melanogaster |
Purification (Comment) | Organism |
---|---|
Phenyl-Sepharose column chromatography and HiTrap Q HP column chromatography | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Drosophila melanogaster | - |
malpighian tubule | - |
Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Drosophila tachykinin-1 + H2O | 3% activity compared to Locusta tachykinin-1 | Drosophila melanogaster | APTSS + FIGMR-amide | - |
? | |
Drosophila tachykinin-1 + H2O | 3% activity compared to Locusta tachykinin-1 | Drosophila melanogaster OregonR | APTSS + FIGMR-amide | - |
? | |
Drosophila tachykinin-2 + H2O | 33% activity compared to Locusta tachykinin-1 | Drosophila melanogaster | APLAFYG + Leu-Arg-amide | - |
? | |
Drosophila tachykinin-2 + H2O | 33% activity compared to Locusta tachykinin-1 | Drosophila melanogaster OregonR | APLAFYG + Leu-Arg-amide | - |
? | |
Drosophila tachykinin-3 + H2O | 1% activity compared to Locusta tachykinin-1 | Drosophila melanogaster | APTG + FTGMR-amide | - |
? | |
Drosophila tachykinin-3 + H2O | 1% activity compared to Locusta tachykinin-1 | Drosophila melanogaster OregonR | APTG + FTGMR-amide | - |
? | |
Drosophila tachykinin-4 + H2O | 8% activity compared to Locusta tachykinin-1 | Drosophila melanogaster | APVNS + FVGMR-amide | - |
? | |
Drosophila tachykinin-4 + H2O | 8% activity compared to Locusta tachykinin-1 | Drosophila melanogaster OregonR | APVNS + FVGMR-amide | - |
? | |
Drosophila tachykinin-5 + H2O | 2% activity compared to Locusta tachykinin-1 | Drosophila melanogaster | APNG + FLGMR-amide | - |
? | |
Drosophila tachykinin-5 + H2O | 2% activity compared to Locusta tachykinin-1 | Drosophila melanogaster OregonR | APNG + FLGMR-amide | - |
? | |
Drosophila tachykinin-6 + H2O | 17% activity compared to Locusta tachykinin-1 | Drosophila melanogaster | QQR + Phe + ADFNSKFVAVR-amide | - |
? | |
Locusta tachykinin-1 + H2O | 100% activity | Drosophila melanogaster | GPSGFYG + Val-amide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NEP2 | - |
Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Drosophila melanogaster |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | activity is drastically reduced below pH 6.5 and above pH 8.0 | Drosophila melanogaster |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0005 | - |
membrane-bound enzyme, in 0.1 M HEPES, 0.01 mM captopril, at pH 7 and 35°C | Drosophila melanogaster | thiorphan | |
0.002 | - |
soluble enzyme, in 0.1 M HEPES, 0.01 mM captopril, at pH 7 and 35°C | Drosophila melanogaster | phosphoramidon | |
0.003 | - |
membrane-bound enzyme, in 0.1 M HEPES, 0.01 mM captopril, at pH 7 and 35°C | Drosophila melanogaster | phosphoramidon | |
0.08 | - |
soluble enzyme, in 0.1 M HEPES, 0.01 mM captopril, at pH 7 and 35°C | Drosophila melanogaster | thiorphan |