BRENDA - Enzyme Database
show all sequences of 3.4.24.B14

Drosophila melanogaster NEP2 is a new soluble member of the neprilysin family of endopeptidases with implications for reproduction and renal function

Thomas, J.E.; Rylett, C.M.; Carhan, A.; Bland, N.D.; Bingham, R.J.; Shirras, A.D.; Turner, A.J.; Isaac, R.E.; Biochem. J. 386, 357-366 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Drosophila melanogaster S2 cells
Drosophila melanogaster
Inhibitors
Inhibitors
Commentary
Organism
Structure
thiorphan
weak inhibition
Drosophila melanogaster
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Drosophila melanogaster
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Drosophila melanogaster
NEP2 plays a role in renal function and spermatogenesis
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Drosophila melanogaster
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
malpighian tubule
-
Drosophila melanogaster
-
testis
expressed in cyst cells that surround the elongating spermatid bundles in adult testis
Drosophila melanogaster
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
locustatachykinin 1 + H2O
-
667494
Drosophila melanogaster
?
-
-
-
?
additional information
NEP2 plays a role in renal function and spermatogenesis
667494
Drosophila melanogaster
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
NEP2
-
Drosophila melanogaster
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Drosophila melanogaster S2 cells
Drosophila melanogaster
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
thiorphan
weak inhibition
Drosophila melanogaster
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Drosophila melanogaster
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Drosophila melanogaster
NEP2 plays a role in renal function and spermatogenesis
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
malpighian tubule
-
Drosophila melanogaster
-
testis
expressed in cyst cells that surround the elongating spermatid bundles in adult testis
Drosophila melanogaster
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
locustatachykinin 1 + H2O
-
667494
Drosophila melanogaster
?
-
-
-
?
additional information
NEP2 plays a role in renal function and spermatogenesis
667494
Drosophila melanogaster
?
-
-
-
-
Other publictions for EC 3.4.24.B14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733906
Marr
Amyloid-beta and Alzheimers di ...
Homo sapiens, Mus musculus
Front. Aging Neurosci.
6
187
2014
-
-
-
-
-
-
4
-
5
-
-
2
-
3
-
-
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8
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4
-
15
-
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4
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2
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8
-
-
4
-
-
-
-
-
-
-
-
-
1
10
10
1
-
-
735280
Takamatsu
Degradation of amyloid beta by ...
Homo sapiens
Stem Cell Res.
13
442-453
2014
-
-
1
-
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-
1
-
3
1
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2
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4
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3
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2
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6
1
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1
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1
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1
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3
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2
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1
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-
717700
Huang
Altered NEP2 Expression and Ac ...
Homo sapiens
J. Alzheimers Dis.
28
433-441
2012
-
1
-
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1
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1
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1
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-
1
-
-
1
-
-
717067
Hafez
Neprilysin-2 is an important b ...
Mus musculus
Am. J. Pathol.
178
306-312
2011
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5
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1
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1
1
-
-
-
694555
Bland
Locomotor and geotactic behavi ...
Drosophila melanogaster
Peptides
30
571-574
2009
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5
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1
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1
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681904
Oh-Hashi
Biosynthesis, processing, traf ...
Mus musculus
Mol. Cell. Biochem.
313
103-111
2008
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1
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5
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2
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1
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2
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4
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5
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2
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692350
Whyteside
Human neprilysin-2 (NEP2) and ...
Homo sapiens
FEBS Lett.
582
2382-2386
2008
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1
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2
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2
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3
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1
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1
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5
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2
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2
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1
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2
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1
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5
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682251
Bland
Expression of NEP2, a soluble ...
Drosophila melanogaster, Drosophila melanogaster OregonR
Peptides
28
127-135
2007
-
-
1
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-
2
-
2
-
2
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2
-
1
1
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-
2
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12
-
1
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1
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1
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-
4
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1
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4
2
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2
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2
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1
1
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2
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-
12
-
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1
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1
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667419
Voisin
The ultimate tryptophan residu ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
335
356-360
2005
-
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1
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4
-
1
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1
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1
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1
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4
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4
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1
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1
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4
-
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-
667494
Thomas
Drosophila melanogaster NEP2 i ...
Drosophila melanogaster
Biochem. J.
386
357-366
2005
-
-
1
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1
-
1
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1
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1
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2
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2
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1
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2
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669280
Voisin
A three-dimensional model of t ...
Rattus norvegicus
J. Biol. Chem.
279
46172-46181
2004
-
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-
11
6
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1
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8
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2
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7
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11
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11
11
6
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8
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7
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670239
Carpentier
Reduced fertility in male mice ...
Mus musculus
Mol. Cell. Biol.
24
4428-4437
2004
-
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3
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1
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1
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-
395498
Boeckmann
The SWISS-PROT protein knowled ...
Mus musculus
Nucleic Acids Res.
31
365-370
2003
-
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1
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1
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1
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-
690798
Rose
Cell-specific activity of nepr ...
Rattus norvegicus
Biochem. J.
363
697-705
2002
-
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1
-
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-
20
6
2
-
-
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-
2
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1
1
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1
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20
-
2
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19
-
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23
-
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-
1
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20
23
6
2
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1
1
-
1
-
-
20
-
-
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19
-
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690691
Ouimet
Neprilysin II: A putative nove ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
271
565-570
2000
-
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1
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1
1
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1
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3
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651958
Ikeda
Molecular identification and c ...
Mus musculus
J. Biol. Chem.
274
32469-32477
1999
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1
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4
-
2
1
2
1
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1
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1
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10
-
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7
1
3
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1
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1
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4
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2
1
2
1
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1
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10
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7
1
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1
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