Cloned (Comment) | Organism |
---|---|
isozymes SEP and SEPDELTA, DNA determination and analysis, transient expression of both isoforms in CHO cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inhibition of the soluble isozyme SEP | Mus musculus | |
additional information | no inhibition by captopril | Mus musculus | |
phosphoramidon | inhibition of the soluble isozyme SEP | Mus musculus | |
thiorphan | inhibition of the soluble isozyme SEP | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | isozyme SEP also is secreted | Mus musculus | - |
- |
membrane | isozymes SEP and SEPDELTA | Mus musculus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc-metalloprotease | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
x * 110000, membrane-bound isozymes SEP and SEPDELTA, SDS-PAGE, x * 126000, soluble isozyme SEP, SDS-PAGE | Mus musculus |
126000 | - |
x * 110000, membrane-bound isozymes SEP and SEPDELTA, SDS-PAGE, x * 126000, soluble isozyme SEP, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Mus musculus | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9JLI3 | 2 isozymes SEP and splicing variant SEPDELTA which lacks 69 base pair nucleotide segment following the transmembrane helix | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | soluble isozyme SEP, and membrane-bound isozyme SEP and SEPDELTA | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | isozymes SEP and SEPDELTA | Mus musculus | - |
brain | isozymes SEP and SEPDELTA | Mus musculus | - |
heart | isozymes SEP and SEPDELTA | Mus musculus | - |
intestine | isozymes SEP and SEPDELTA | Mus musculus | - |
kidney | isozymes SEP and SEPDELTA | Mus musculus | - |
liver | isozymes SEP and SEPDELTA | Mus musculus | - |
lung | isozymes SEP and SEPDELTA | Mus musculus | - |
additional information | in most cases isozyme SEPDELTA shows higher expression rates than isozyme SEP | Mus musculus | - |
ovary | isozyme SEP | Mus musculus | - |
testis | isozyme SEP, very low amount of isozyme SEPDELTA | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin I + H2O | - |
Mus musculus | ? | - |
? | |
atrial natriuretic peptide + H2O | - |
Mus musculus | ? | - |
? | |
bradykinin + H2O | - |
Mus musculus | ? | - |
? | |
endothelin-1 + H2O | - |
Mus musculus | ? | - |
? | |
protein + H2O | - |
Mus musculus | peptides | - |
? | |
protein + H2O | broad substrate specificity | Mus musculus | peptides | - |
? | |
Substance P + H2O | - |
Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 110000, membrane-bound isozymes SEP and SEPDELTA, SDS-PAGE, x * 126000, soluble isozyme SEP, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
M13.008 | Merops-ID | Mus musculus |
More | may be identical with neprilysin EC 3.4.24.11 | Mus musculus |
SEP | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
sharp pH-dependence | Mus musculus |