Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.B12 extracted from

  • Vankemmelbeke, M.N.; Holen, I.; Wilson, A.G.; Ilic, M.Z.; Handley, C.J.; Kelner, G.S.; Clark, M.; Liu, C.; Maki, R.A.; Burnett, D.; Buttle, D.J.
    Expression and activity of ADAMTS-5 in synovium (2001), Eur. J. Biochem., 268, 1259-1268.
    View publication on PubMed
Search for more literature for 3.4.24.B12

Cloned(Commentary)

Cloned (Comment) Organism
expression of FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells via baculovirus infection, secretion of recombinant protein to the medium Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
heparin
-
 Bos taurus
heparin
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-
extracellular
-
 Bos taurus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aggrecan + H2O Homo sapiens enzyme may play a role in cartilage breakdown ?
-
 ?
aggrecan + H2O Bos taurus enzyme may play a role in cartilage breakdown ?
-
 ?
proteins + H2O Homo sapiens
-
 peptides
-
 ?
proteins + H2O Bos taurus
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus Q9TT92
-
-
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification cleavage by furin Homo sapiens
proteolytic modification cleavage by furin Bos taurus

Purification (Commentary)

Purification (Comment) Organism
recombinant FLAG-tagged from insect Sf9 cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cartilage
-
 Homo sapiens
-
synovial membrane
-
 Bos taurus
-
synovium
-
 Bos taurus
-
synovium arthritic Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aggrecan + H2O substrate from cartilage, extensive digestion, N-terminal cleavage sites between glutamate and small uncharged aliphatic amino acids in nonglycosylated regions of the aggregan core protein: the interglobular domain E373-A, and between chondroitin sulfate attachment sites 1 and 2 E1480-G Homo sapiens ?
-
 ?
aggrecan + H2O substrate from cartilage, N-terminal cleavage sites between glutamate and small uncharged aliphatic amino acids in nonglycosylated regions of the aggregan core protein: the interglobular domain E373-A, and within the chondroitinsulfate 2 attachment region E1666-G, E1771-A, and E1871-L Bos taurus ?
-
 ?
aggrecan + H2O enzyme may play a role in cartilage breakdown Homo sapiens ?
-
 ?
aggrecan + H2O enzyme may play a role in cartilage breakdown Bos taurus ?
-
 ?
additional information interaction with sulfated glucosaminglycan may be very important for the localization and activity of the enzyme Bos taurus ?
-
 ?
additional information interaction with sulfated glucosaminglycan may be very important for the localization and activity of the enzymew Homo sapiens ?
-
 ?
proteins + H2O
-
 Homo sapiens peptides
-
 ?
proteins + H2O
-
 Bos taurus peptides
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
ADAMTS-5
-
 Homo sapiens
ADAMTS-5
-
 Bos taurus
aggrecanase-2
-
 Homo sapiens
aggrecanase-2
-
 Bos taurus
M12.225 Merops-ID Homo sapiens
M12.225 Merops-ID Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Bos taurus