Cloned (Comment) | Organism |
---|---|
expression of full-length wild-type enzyme in human embryonic kidney cell line 293T, expression of C-terminal region of the enzyme, corresponding to amino acid residues 549-951, in breast tumor derived cell line T47D | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | introduction of a KpnI site at the 5'-end, expression in carcinoma cell line T47D | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | metalloprotease | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | vascular endothelial growth factor 165 and vascular endothelial growth factor 165 receptor tyrosine kinase are no substrates for proteolytic cleavage, but the enzyme blocks endothelial vascularization by inhibiting the necessary phosphorylation of the receptor, interaction with heparin via the first TSP1 domain at the C-terminal part, enzyme shows an antiangiogenic effect, the enzyme is able to shed proteins from the surface of the endothelium | ? | - |
? | |
proteins + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from cell line 293T | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | vascular endothelial growth factor 165 and vascular endothelial growth factor 165 receptor tyrosine kinase are no substrates for proteolytic cleavage, but the enzyme blocks endothelial vascularization by inhibiting the necessary phosphorylation of the receptor | Homo sapiens | ? | - |
? | |
additional information | vascular endothelial growth factor 165 and vascular endothelial growth factor 165 receptor tyrosine kinase are no substrates for proteolytic cleavage, but the enzyme blocks endothelial vascularization by inhibiting the necessary phosphorylation of the receptor, interaction with heparin via the first TSP1 domain at the C-terminal part, enzyme shows an antiangiogenic effect, the enzyme is able to shed proteins from the surface of the endothelium | Homo sapiens | ? | - |
? | |
proteins + H2O | - |
Homo sapiens | peptides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADAMTS1 | - |
Homo sapiens |
M12.222 | Merops-ID | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Homo sapiens |