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Literature summary for 3.4.24.B11 extracted from

  • Luque, A.; Carpizo, D.R.; Iruela-Arispe, M.L.
    ADAMTS1/METH1 inhibits endothelial cell proliferation by direct binding and sequestration of VEGF165 (2003), J. Biol. Chem., 278, 23656-23665.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of full-length wild-type enzyme in human embryonic kidney cell line 293T, expression of C-terminal region of the enzyme, corresponding to amino acid residues 549-951, in breast tumor derived cell line T47D Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information introduction of a KpnI site at the 5'-end, expression in carcinoma cell line T47D Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ metalloprotease Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens vascular endothelial growth factor 165 and vascular endothelial growth factor 165 receptor tyrosine kinase are no substrates for proteolytic cleavage, but the enzyme blocks endothelial vascularization by inhibiting the necessary phosphorylation of the receptor, interaction with heparin via the first TSP1 domain at the C-terminal part, enzyme shows an antiangiogenic effect, the enzyme is able to shed proteins from the surface of the endothelium ?
-
?
proteins + H2O Homo sapiens
-
peptides
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from cell line 293T Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information vascular endothelial growth factor 165 and vascular endothelial growth factor 165 receptor tyrosine kinase are no substrates for proteolytic cleavage, but the enzyme blocks endothelial vascularization by inhibiting the necessary phosphorylation of the receptor Homo sapiens ?
-
?
additional information vascular endothelial growth factor 165 and vascular endothelial growth factor 165 receptor tyrosine kinase are no substrates for proteolytic cleavage, but the enzyme blocks endothelial vascularization by inhibiting the necessary phosphorylation of the receptor, interaction with heparin via the first TSP1 domain at the C-terminal part, enzyme shows an antiangiogenic effect, the enzyme is able to shed proteins from the surface of the endothelium Homo sapiens ?
-
?
proteins + H2O
-
Homo sapiens peptides
-
?

Synonyms

Synonyms Comment Organism
ADAMTS1
-
Homo sapiens
M12.222 Merops-ID Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Homo sapiens