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Literature summary for 3.4.24.B10 extracted from
- Regulation of human ADAM 12 protease by the prodomain. Evidence for a functional cysteine switch (1999), J. Biol. Chem., 274, 13427-13433.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transient expression in COS-7 cells of the enzyme mutants, secretion to the medium | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C179A | site-directed mutagenesis, exchange of the cysteine-switch residue of the prodomain, still active in complex formation with alpha2-macroglobulin | Homo sapiens |
| C179H | site-directed mutagenesis, exchange of the cysteine-switch residue of the prodomain, still active in complex formation with alpha2-macroglobulin | Homo sapiens |
| C179H/E351Q | site-directed mutagenesis, exchange of the cysteine-switch residue of the prodomain results in a no longer latent but inactive zymogen | Homo sapiens |
| C179S | site-directed mutagenesis, exchange of the cysteine-switch residue of the prodomain the glutamic acid residue and of the zinc binding domain involved in catalytic activity, inactive to form complexes with alpha2-macroglobulin, results in a no longer latent but inactive zymogen | Homo sapiens |
| additional information | KR207NG mutation prevents cleavage of the prodomain by furin, construction of enzyme mutants where the prodomain or the metalloprotease domain or both are eliminated or exchanged for an Ig kappa-chain leader sequence, secretion to the medium, inactive mutant | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Homo sapiens |  |
| hydroxamate inhibitor BB-94 | slight inhibition | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | metalloprotease domain is the catalytically active site | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | enzyme complexes alpha2-macroglobulin | ? | - |
? | |
| protein + H2O | Homo sapiens | - |
peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O43184 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | degylcosylation with endoglycosidase H | Homo sapiens |
| proteolytic modification | the cysteine-switch of the prodomain maintaines the zymogen in a latent state, enzyme is synthesized as a catalytically inactive zymogen, in vivo cleavage by a furin-like endopeptidase, autoactivation is possible with N-ethylmaleimide | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme complexes alpha2-macroglobulin | Homo sapiens | ? | - |
? | |
| protein + H2O | - |
Homo sapiens | peptides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| a disintegrin and metalloproteinase domain 12 | - |
Homo sapiens |
| ADAM12 | - |
Homo sapiens |
| M12.212 | Merops-ID | Homo sapiens |
| meltrin alpha | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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