Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.B10 extracted from

  • Loechel, F.; Wewer, U.M.
    Activation of ADAM 12 protease by copper (2001), FEBS Lett., 506, 65-68.
    View publication on PubMed
Search for more literature for 3.4.24.B10

Cloned(Commentary)

Cloned (Comment) Organism
expression of the soluble enzyme form ADAM 12-S in COS cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C179S site-directed mutagenesis, mutant behaves identical to the wild-type concerning metal ions, therfore the cysteine-switch and activation by copper are 2 different components of enzyme activation Homo sapiens
additional information elimination of the cysteine-switch by a point mutation in the propeptide has no effect on copper activation, whereas mutation of an unpaired cysteine residue in the catalytic domain resulted in a mutant form of ADAM 12-S that is insensitiv to copper Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ activates, dependent on, multistep activation mechanism involving both furin cleavage and copper binding Homo sapiens
Zn2+ activates, dependent on, enzyme contains a catalytically active zinc-binding domain Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Homo sapiens
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O43184
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification autocleavage occurs near the junction of the prodomain and the catalytic domain upon inactivation of cysteine residues C179 and in presence of Cu2+ which is further promoted in presence of ascorbate, multistep activation mechanism involving both furin cleavage and copper binding Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant from COS cells Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
proteolysis of proteins active site zinc ion is coordinated by Cys179 Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protein + H2O
-
 Homo sapiens peptides
-
 ?

Synonyms

Synonyms Comment Organism
a disintegrin and metalloprotease-12
-
 Homo sapiens
ADAM 12-S
-
 Homo sapiens
M12.212 Merops-ID Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens