Crystallization (Comment) | Organism |
---|---|
crystal structures of Zmp1 in its unbound and peptide-bound forms. The structure analysis reveals a fold similar to Bacillus anthracis lethal factor. The structure consists of an upper N-terminal domain and a lower helical C-terminal domain with respect to the active-site helix aalpha4, which harbors two of the three zinc-binding residues His142 and His146 and the catalytic base Glu143 in the typical metalloprotease motif (H142E143TAH146) | Clostridioides difficile |
Protein Variants | Comment | Organism |
---|---|---|
E143A | 18% of wild-type activity | Clostridioides difficile |
E143A/Y178F | complete loss of activity | Clostridioides difficile |
Y178F | 41% of wild-type activity | Clostridioides difficile |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Clostridioides difficile | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Clostridioides difficile |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridioides difficile | Q183R7 | - |
- |
Clostridioides difficile 630 | Q183R7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-EVNPPVPD + H2O | - |
Clostridioides difficile | acetyl-EVNP + PVPD | - |
? | |
acetyl-EVNPPVPD + H2O | - |
Clostridioides difficile 630 | acetyl-EVNP + PVPD | - |
? | |
fibrinogen beta-chain + H2O | almost complete digestion within 1 h | Clostridioides difficile | ? | - |
? | |
fibrinogen beta-chain + H2O | almost complete digestion within 1 h | Clostridioides difficile 630 | ? | - |
? | |
Fibronectin + H2O | - |
Clostridioides difficile | ? | poor substrate | ? | |
Fibronectin + H2O | - |
Clostridioides difficile 630 | ? | poor substrate | ? |
Synonyms | Comment | Organism |
---|---|---|
Zmp1 | - |
Clostridioides difficile |